RETINAL GLUTAMATE IN EXPERIMENTAL GLAUCOMA

描述：(摘自摘要)调查员打算探索 原发性开角区视网膜神经节细胞死亡的潜在基础 青光眼。她的调查是基于之前的工作，该工作表明 青光眼患者玻璃体中的谷氨酸含量异常高。 她打算首先探索玻璃体谷氨酸与 注意力集中和青光眼损害。这将由天然气进行。 猕猴双眼的色质联用研究 单眼实验诱导的青光眼。她打算将 谷氨酸升高的幅度与神经纤维丢失的程度有关。 其次，她提出了一种假设，即玻璃体的高度 谷氨酸是米勒细胞内扰动的结果。视网膜 众所周知，穆勒细胞在从体内清除谷氨酸的过程中发挥着重要作用 细胞外空间，以及随后的新陈代谢。假说 因此提出，玻璃体谷氨酸水平升高可能是 这是穆勒细胞活性改变的结果。她打算带着 Out免疫细胞化学分析评估Muller细胞的变化 谷氨酸代谢的活性。希望根据这些发现， 将会找到一个解释为什么谷氨酸水平在 玻璃体，以及确定潜在的新药理药剂。

DESCRIPTION: (from the abstract) The investigator intends to explore the underlying basis of retinal ganglion cell death seen in primary open angle glaucoma. Her investigations are based on previous work that has suggested that glutamate is abnormally high in the vitreous of patients with glaucoma. She intends to explore first the relationship between vitreal glutamate concentration and glaucomatous damage. This will be carried out by gas chromatography/mass spectroscopy in the paired eyes of monkeys with monocular experimentally induced glaucoma. She intends to correlate the magnitude of the glutamate elevation with the degree of nerve fiber loss. Secondly, she presents the hypothesis that the elevation in vitreal glutamate is a consequence of a perturbation in Muller cells. Retinal Muller cells are known to play a major role in the removal of glutamate from the extracellular space, and in its subsequent metabolism. The hypothesis is therefore presented that the elevated vitreal glutamate level can be the result of alterations in the activity of Muller cells. She intends to carry out immunocytochemical analyses to assess alterations in Muller cell activity in glutamate metabolism. It is hoped, based on these findings, that an explanation will be found for the elevated level of glutamate in the vitreous, as well as identifying potential new pharmacologic agents.