MECHANISM OF NERVE GROWTH CONE TURNING AND BRANCHING

这项研究的目的是调查生长机制的差异 通过检查生长锥体向正指导线索的锥体移动 转向神经营养因子(NGF、BDNF、 新生大鼠、成年大鼠和老年大鼠。 我们将记录生长锥体的行为以及它们的接近 神经营养因子。在固定和贴上标签后，我们将 定位微管并作用于细丝并分析其 视频显微镜图像。我们将应用药物来确定其作用 神经营养因子在这种反应中的作用机制及鉴定 涉及受体的敏感性。我们将调查以下一些步骤 调节转弯和分支的信号转导事件 指向NGF的点源(钙离子内流，trk的激活 信号级联、TrkA和PI-Kinase的自动磷酸化) 因为他们在试验组中的差异。 这些实验的结果将澄清两者之间的区别 新生、成年和老年大鼠神经元的再生能力 以及分子水平上的细胞再生过程。会的 阐明神经组织再生的精细过程和 外周神经元再生和修复轴突的能力 因不同疾病而受伤或受损的通路。

The study aims to investigate the differences in the mechanism of growth cone migration to a positive guideline cue by examination of growth cone turning and branching toward a point of neurotrophic factors (NGF,BDNF, NT-3) in neunatal, adult and old rats. We will record the behaviour of growth cones as well as their approach to neurotrophic factors. After fixing and labeling them, we shall localize the microtubules and act in filaments and analize their videomicroscopic images. We will apply drugs to determine the role of neurotrophic factors in the mechanisms of this response and identify the sensitivity of involving receptors. We will investigates some steps of signal transduction events, that mediates the turning and branching toward a point source of NGF (the influx of Ca++, activation of trk signaling cascade, autophosphorialation of trkA and PI-kinase), as well as their differences in the experimental groups. The results of these experiments would clarify the difference between the regenerative capacity of neurons from neonatal, adult and aged rats and the processes of cell regeneration at molecular level. It will clarify the fine processes of the nervous tissue regeneration and the abilities of the peripheral neurons to regenerate and repair axonal pathways that are injured or damaged by different disease.