HIGH THROUGHPUT METHOD TO IDENTIFY IMMUNOGENIC MOLECULES

描述：（申请人的描述）最广泛和迄今为止最 鉴定肿瘤细胞共有免疫原性分子的成功方法 筛选具有肿瘤特异性CTL的cDNA文库。 适用本 该策略导致鉴定了表达的几个基因家族， 主要是在人类黑素瘤中。 然而，有两个主要的限制 to this approach方法. 首先，筛选方法是非常劳动密集型的， 实践中，这限制了给定库中可以 筛选出 其次，除了肾细胞癌， 肿瘤特异性细胞毒性T细胞很难从 肿瘤浸润淋巴细胞（TIL）或PBL的患者， 比黑色素瘤，特别是上皮细胞癌的肿瘤类型 占人类肿瘤的80%以上

DESCRIPTION: (Applicant's Description) The most widespread and to date most successful approach to identify immunogenic molecules common to tumor cells is to screen a cDNA library with tumor-specific CTL. Application of this strategy has led to identification of several gene families expressed predominantly in human melanoma. There are, however, two major limitations to this approach. First, screening methods are very labor intensive and, in practice, this limits the number of elements in a given library that can be screened. Second, with the possible exception of renal cell carcinoma, tumor-specific cytotoxic T cells have been very difficult to isolate from either tumor infiltrating lymphocytes (TIL) or PBL of patients with other types of tumors than melanoma, especially the epithelial cell carcinomas that comprise greater than 80% of human tumors.