REGULATION OF UROCORTIN TISSUE SPECIFIC EXPRESSION

促肾上腺皮质激素释放因子（CRF）， 下丘脑-垂体-肾上腺皮质（HPA）轴，也是一个 在协调自主神经，免疫和 对压力的行为反应 最近，一种新的CRF样肽， 鉴定出尿皮质素（UCN）。 其受体结合亲和力和 不同的组织分布表明达特它具有独特的发育 和生理作用。 我们建议研究组织特异性 UCN的表达式。 将为UCN分离基因组序列 基因并与报告基因相连。 表达UCN的细胞系和 组织将被用作模型系统来分析UCN 转录元件，指导组织特异性表达 报告基因。 转录与转录之间的相互作用 然后将研究元素及其反式作用因子， 揭示了哪些因素通过这些元素起作用。 新奇因素 将被克隆并进一步分析。 组织的作用 还将使用转基因技术确认特定元件。

Corticotropin-releasing factor (CRF), a key regulator of hypothalamus-pituitary-adrenal cortical (HPA) axis, is also a critical factor in coordinating autonomic, immunological and behavioral responses to stress. Recently, a novel CRF-like peptide, urocortin (Ucn) was identified. Its receptor binding affinity and distinct tissue distribution suggests tat it has unique developmental and physiological roles. We propose to study the tissue-specific expression of Ucn. The genomic sequence will be isolated for the Ucn gene and linked to a reporter gene. Ucn-expressing cell lines and tissues will be used as a model system to analyze the Ucn transcription elements that direct the tissue-specific expression of the reporter gene. The interaction between the transcription elements and their trans-acting factors will then be studied to reveal what factors act through these elements. Novel factors identified will be cloned and further analyzed. The role of tissue specific elements will also be confirmed using transgenic techniques.