Core--Immunology

Immunology Core将进行多参数流式细胞术测定和细胞因子测定。Kamoun博士将担任该核心的主任。他是宾夕法尼亚大学医学中心临床免疫学实验室主任。临床免疫学实验室为患有自身免疫性疾病（包括MS和SLE）的患者提供广泛的实验室检测，以及先进的多参数四色流式细胞术检测。可以在用抗体疗法治疗的MS和SLE患者中确定CD 4+和CD 8 + T细胞和B细胞亚群的改变。可以通过评估一系列活化标志物和粘附分子的CD 4+、CD 8+或B细胞的表达来评价淋巴细胞活化。通过透化细胞的细胞内染色，可以使用我们实验室最近建立的全血单细胞细胞因子测定来分析组成性和诱导的细胞因子产生。该方法允许鉴定产生细胞因子的细胞，同时分析表面表型。细胞因子的体外产生也可以使用基于ELISA的测定来测量。我们可以测量IL-2、IL-4、IL-10、IL-12（p40和生物活性p70异源二聚体）、INF-γ、TNF-α和LT-α。实验室使用的所有试剂在使用前均经过质量控制程序。质量控制程序的所有结果都由主管进行审查，并在必要时采取纠正措施。我们的实验室参与QA计划，包括CAP季度调查和CDC T细胞免疫表型性能评估计划。每天使用微珠悬浮液监测流式细胞仪的性能，以证明散射、荧光和补偿参数在规定限度内。在实验室的这一部分雇用的技术人员都有资格根据CLIA 88准则进行“高复杂性测试”。实验室主任和主管分别满足CLIA 88对主任和技术主管的要求。实验室为所有员工制定了全面的安全计划，包括OSHA法规，并符合所有联邦、州和地方法规。

The Immunology Core will perform multi-parameter flow cytometry assays and cytokine assays. Dr. Kamoun, will be the Director of this core. He is the Director of the Clinical Immunology Laboratory at the University of Pennsylvania Medical Center. The Clinical Immunology Laboratory provides a wide range of laboratory tests on patients with autoimmune diseases including MS, and SLE as well as advanced multi- parameter four-color flow cytometry-based assays. Alterations in subset of CD4+ and CD8+ T cells and B cells can be determined in MS and SLE patients treated with antibody therapies. Lymphocyte activation can be evaluated assessing expression CD4+, CD8+ or B cells of a series of activation markers and adhesion molecules. Analysis of constitutive and induced cytokine production by intracellular staining of permeabilized cells can be performed using whole blood single cell cytokine assays which was recently established in our laboratory. This method allows the identification of cells producing cytokine while simultaneously analyzing the surface phenotype. In vitro production of cytokine can also be measured using ELISA-based assays. We can measured IL-2, IL-4, IL-10, IL-12 (p40 and the biologically active p70 heterodimer), INF-gamma, TNF-alpha and LT-alpha. All reagents used in the Laboratory are subjected to quality control procedures prior to use. All results of QC procedures are reviewed by the supervisor with corrective action implemented as necessary. Our lab participates in QA programs including the CAP surveys quarterly and in the CDC T cell immunophenotyping performance evaluation program. Performance of the flow cytometers is monitored daily using a suspension of beads to demonstrate scatter, fluorescence, and compensation parameters within defined limits. The Technologists employed in this section of the laboratory are each qualified under CLIA88 guidelines to perform "high complexity testing". The Laboratory Director and the Supervisor meet CLIA 88 requirements as director and technical supervisor respectively. The laboratory maintains a comprehensive safety program for all employees encompassing OSHA regulation and conforming to all Federal, State and local regulation.