NEUROTROPHINS AND VISCERAL AFFERENT NEURONS IN DIABETES

糖尿病自主神经病变改变自主神经反射和IS 与糖尿病患者的发病率和预后不良有关。 这种功能障碍的一部分是由于内脏传入的改变。 神经元。迷走神经内脏传入神经元损伤 舌咽神经扰乱重要的心血管、呼吸系统 还有胃肠反射。这个项目的目标是 更好地描述糖尿病引起的内脏传入改变 并研究神经营养因子家族的作用。 神经营养因子与内脏传入神经功能/功能障碍 糖尿病。研究人员将检验糖尿病的假说 诱导代谢、神经化学、神经病理、微血管和 内脏传入神经元的功能改变 通过适当的神经营养支持缓解。他们将使用 链脲佐菌素治疗糖尿病大鼠(有/无胰岛素替代) 糖尿病引起的内脏传入神经改变的特征 和它们的神经节在实验开始后的不同时间 糖尿病。为了确定神经营养因子作用的损害是否可能 参与糖尿病引起的内脏传入改变 神经元，他们将评估神经营养因子是否传递到 内脏传入神经元受外周逆行改变的影响 糖尿病患者的运输和/或神经营养因子水平的改变，以及 糖尿病对神经营养素受体存在的影响。最后， 特异性神经营养因子缓解糖尿病的作用 糖尿病患者内脏传入神经元的改变和影响 下定决心。

Diabetic autonomic neuropathy alters autonomic reflexes and is associated with morbidity and a poor prognosis for diabetic patients. A component of this dysfunction is due to altered visceral afferent neurons. Injury to visceral afferent neurons of the vagus and glossopharyngeal nerves disrupts important cardiovascular, respiratory and gastrointestinal reflexes. The objectives for this project are to better characterize diabetes induced alterations in visceral afferent neurons, and to study the actions of the neurotrophin family of neurotrophic factors in visceral afferent nerve function/dysfunction in diabetes. The investigators will test the hypotheses that diabetes induces metabolic, neurochemical, neuropathologic, microvascular and functional alterations in visceral afferent neurons that can be alleviated with appropriate neurotrophic support. They will use streptozotocin treated diabetic rats (with/without insulin replacement) to characterize diabetes induced alterations in visceral afferent nerves and their ganglia at various times after initiation of experimental diabetes. To determine whether an impairment of neurotrophin actions may be involved in diabetes induced alterations in visceral afferent neurons, they will assess whether the delivery of neurotrophins to visceral afferent neurons is affected by altered retrograde external transport and/or by altered levels of neurotrophins in diabetes, and the effect of diabetes on the presence of neurotrophin receptors. Finally, the ability of specific neurotrophins to alleviate diabetes induced alterations and to affect visceral afferent neurons in diabetes will be determined.