GENETIC MONITORING OF RODENTS

本合同的目的是提供遗传监测资源给 确保F344大鼠、B6C3F1等近交系种群的遗传完整性 为毒理学和其他研究生产的小鼠和特殊菌株 以及NTP和NlEHS的测试程序。最多20个指定地点将 通过各种不同的电泳法对每个品系或杂交品种进行监测 组织匀浆和血清蛋白。杂交后代的亲本品系， 杂交种和自交系的建立、推广和生产 每隔一段时间将对群体进行遗传完整性评估。 近亲交配啮齿动物的遗传完整性也将通过皮肤移植进行评估。 适当时，限制性片段长度多态(RFLP)和 聚合酶链式反应的DNA简单序列长度多态(SSLP)可能是 评估以确定遗传变异。测试的遗传完整性 动物是开发可靠和准确的研究数据的关键 动物实验。对地基和生产的持续监测 为研究提供的群体和动物将确保来自 NTP和NIEHS动物研究将从基因同质性 啮齿动物。实验动物的遗传同质性对于 不同项目和方案背景研究的比较 损伤，检测设施之间的损伤，化学或化学或 物理代理和同一实验室内的加班。

The purpose of this contract is to provide genetic monitoring resource to assure the genetic integrity of inbred stock such as F344 rats, B6C3F1 mice and special strains produced for the toxicology and other research and testing programs of the NTP and NlEHS. Up to 20 designated loci will be monitored for each strain or hybrid by electrophoresis of various tissue homogenates and serum proteins. Parental strains of hybrids, hybrids and inbred strains from the foundation, expansion and production colonies will be evaluated for genetic integrity at periodic intervals. Genetic integrity of inbred rodents will be evaluated by skin grafts also. When appropriate, restriction fragment length polymorphisms (RFLPs) and simple sequence length polymorphisms (SSLPs) of DNA by PCR may be evaluated to identify genetic variance. The genetic integrity of the test animals is essential for developing reliable and accurate research data in animal experiments. Continuous monitoring of foundation and production colonies and animals supplied for studies will ensure that data from the NTP and NIEHS animal studies will be collected from genetically homogenous rodents. Genetic homogeneity of test animals will be essential for comparison of studies from different projects and programs for background lesions, lesions between testing facilities, lesions between chemical or physical agents and within the same laboratory overtime.