DIETARY EFFECTS ON MEMBRANE LIPIDS

描述(改编自申请人的摘要)：长远目标 这项建议是为了了解膜脂在形成和发展中的作用。 膜细胞器的利用。对照组雄性小鼠分泌溶酶体酶 和多板层小体从肾近端小管细胞进入尿液。 这些身体富含含有C22：6-n-3脂肪的磷脂酰胆碱。 必须从饮食中提取的酸。多板层小体的分泌物 溶酶体酶在人类Chediak-Higashi综合征和 米色小鼠突变体。这导致了一种工作假设，即这种类脂起到了一些 在导致这些溶酶体尿液排泄的分泌过程中的作用 材料。这项提议的具体目的是调查其影响 膳食脂质(二十二碳六烯酸，C22：6N-3)对 对照组和Chediak-Higashi CHS小鼠的溶酶体样多板层小体。 研究人员建议：(I)确定饮食中的n-3脂肪酸水平 溶酶体酶和多板层小体对尿液分泌的影响 控制雄性小鼠，以及(Ii)确定饮食中n-3脂肪酸水平 对米色突变雄性小鼠尿液分泌的影响。

DESCRIPTION (adapted from the applicant's abstract): The long-range goal of this proposal is to understand the role of membrane lipids in the formation and utilization of membrane organelles. Control male mice secrete lysosomal enzymes and multilamellar bodies from the renal proximal tubule cells into the urine. These bodies are enriched in phosphatidylcholine containing C22:6-n-3 fatty acid that must be derived from the diet. The secretion of multilamellar bodies and lysosomal enzymes is blocked in human Chediak-Higashi syndrome and in the beige mouse mutant. This led to a working hypothesis that this lipid plays some role in the secretory process leading to urinary excretions of these lysosomal materials. The specific aims of this proposal are to investigate the influence of dietary lipids (docosahexaenoic acid, C22:6n-3) on the formation of lysosomal-like multilamellar bodies in control and Chediak-Higashi CHS) mice. The investigator proposes to (i) Determine if n-3 fatty acid levels in the diet affect urinary secretion of lysosomal enzymes and multilamellar bodies in control male mice, and (ii) Determine if n-3 fatty acid levels in the diet affect the block to urinary secretion in beige mutant male mice.