DEVELOPMENTAL ROLE OF (TAG 1) IN TRANSGENIC ZEBRAFISH

描述(改编自调查人员摘要)：本提案旨在 研究TAG I(瞬时表达轴突糖蛋白)在体内的作用 通过对转基因斑马鱼(Danio rerio That 可以在发育的特定阶段错误表达这种分子。 TAG I是免疫球蛋白超家族的一种细胞黏附分子 通过胚胎细胞的子集瞬时表达，并作为可育的 体外培养轴突生长的基质。它的表达方式在 分化的神经元表明它参与了轴突的生长过程， 选择性分束、靶标识别和细胞迁移。虽然 斑马鱼Tag I的同源物已在大鼠(Tag-1)、雏鸡(Axin)中克隆 1)和人类(TAX-1)，该分子在体内的作用有限 分析。斑马鱼代表了一个理想的模型系统来研究 TAG I的功能，因为它是一种脊椎动物胚胎，能够适应 各种细胞、分子和基因技术。这项建议旨在 1)构建与绿色相关的斑马鱼Tag I转基因 荧光蛋白(GFP)和由斑马鱼驱动的休克启动子；2) 创建稳定的含有这些构建物的转基因系；以及3)分析 在发育的不同阶段错误表达TAG I的后果。一个 更好地理解TAG I在神经元发育中所起的确切作用 可能会增加我们对这些复杂联系的理解 神经系统的建立，以及建议的治疗手段 神经损伤后的干预。通过这一领域的赠款提供资金将使 为本科生提供独特的学习体验，方便校长 调查员向独立调查员的转变。

DESCRIPTION (adapted from investigator's abstract): This proposal seeks to investigate the in vivo role of tag I (transiently expressed axon glycoprotein) through the generation and analysis of transgenic zebrafish (Danio rerio that can be produced to misexpress this molecule at specific stages of development. tag I is a cell adhesion molecule of the immunoglobulin superfamily that is expressed transiently by a subset of embryonic cells and serves as a fertile substrate for neurite outgrowth in vitro. Its pattern of expression in the differentiating neurons has implicated it in the processes of axon outgrowth, selective fasciculation, target recognition and cell migration. Although homologs of zebrafish tag I have been cloned in the rat (TAG-1), chick (axonin 1), and human(TAX-1), the in vivo role of this moIecule has seen limited analysis. The zebrafish represents an ideal model system to investigate the function of tag I because it is a vertebrate embryo that is amenable to a variety of cellular, molecular and genetic techniques. This proposal seeks to 1) construct zebrafish tag I transgenes that have been linked to green fluorescent protein(GFP) and are driven by the zebrafish at shock promoter; 2) create stable transgenic lines harboring these constructs; and 3) analyze the consequences of misexpressing tag I at different stages of development. A better understanding of the precise role tag I plays in neuronal development could increase our understanding of how the intricate connections of the nervous system are established, as well as suggest means for therapeutic intervention after neuronal injury. Funding through this AREA grant will allow unique learning experiences for undergraduates and facilitate the principal investigator's transition to an independent investigator.