NEURAL DEVELOPMENT AND GENETICS OF ZEBRAFISH

建议为高级研究生开设为期两周的强化课程， 医学生、博士后研究员、医学研究员和 独立调查人员将专注于发展和 斑马鱼的遗传学，特别强调神经系统。这个 课程的第一周将涵盖早期发育和遗传学。 斑马鱼和相关方法学，而第二周 将专注于神经系统。上午将专门用来讲课 和讨论，下午到3-4小时的实验室练习。每个人 DAY将在一名高级教员的监督下进行 得到一到两名初级教员的协助。这门课程将 以12-16名学生为限。 第一周的课程将涵盖儿童的发展阶段 斑马鱼，化学诱变和插入突变，早期发育和 器官发生与斑马鱼养殖。在第二周，学生们 会被引入斑马鱼的神经系统，它的发育 以及神经系统突变的分析。待确定的具体领域 第二周的内容包括前脑和后脑、脊髓、 眼睛和视网膜、顶盖和斑马鱼行为的各个方面。实验室 第一周的练习将向学生介绍野生动物的发育。 类型胚胎和突变胚胎、核酸注射技术、细胞 移植、原位杂交和单个细胞的标记 细胞。在第二周，学生们将对紧张的斑马鱼进行解剖 系统，研究脑组织学，对野生型和 突变动物，并学习轴突寻路技术。

An intensive two-week course is proposed for advanced graduate students, medical students, postdoctoral fellows, medical research fellows and independent investigators that will focus on the development and genetics of zebrafish with special emphasis on the nervous system. The first week of the course will cover the early development and genetics of zebrafish and the relevant methodologies, whereas the second week will focus on the nervous system. Mornings will be devoted to lectures and discussion and afternoons to 3-4 hour laboratory exercises. Each day will be under the supervision of a senior faculty member who will be assisted by one or two more junior faculty members. The course will be limited to 12-16 students. Lectures in the first week will cover the developmental stages of zebrafish, chemical and insertional mutagenesis, early development and organogenesis and zebrafish husbandry. In the second week, the students will be introduced to the nervous system of zebrafish, its development and the analysis of nervous system mutants. Specific areas to be covered in the second week include the fore- and hindbrain, spinal cord, eye and retina, tectum and aspects of zebrafish behavior. Laboratory exercises in the first week will introduce students to developing wild- type and mutant embryos, techniques of nucleic acid injection, cell transplantation, in situ hybridization, and the labeling of single cells. In the second week, students will dissect the zebrafish nervous system, study brain histology, perform behavioral tests on wild-type and mutant animals, and learn axonal pathfinding techniques.