PULMONARY OXYGEN TOXICITY--MECHANISMS AND INTERVENTIONS

申请人是妇产科、儿科的助理教授， 药理学，在生物学方面有相当丰富的经验， 肺表面活性剂系统和表面活性剂替代疗法。的 申请人的长期职业目标是发展多学科 一个研究项目，这将使他联合收割机的利益， 肺细胞生物学与实验治疗方法 急性肺疾病的治疗。 候选人更直接的目标 是为了扩大他的实验室目前的细胞生物学专业知识， 在分子生物学领域获得更多的实践经验。 的 RCDA将允许申请人减少他目前的教学， 行政管理职责，以便将全部时间用于 实验室，实现这些目标。 申请人所属院校为 支持他的目标，并同意候选人对如何 来实现它们。 有一些国家卫生研究院资助的高级教师在 纽约的州立大学，对肺部研究感兴趣， 与申请人合作，并已形成一个肺 研究小组，以促进沟通。 此外，大学 正在建造一座新的研究大楼，预计于 1992-93年，这将为调查小组提供广阔的空间 有着共同的兴趣。 最后，申请人具有广泛的 与罗切斯特大学合作，并已被邀请 花时间在斯图尔特·霍洛维茨博士的实验室里， 获得分子生物学方面的培训。 为该奖项提出的研究 期间涉及负责细胞生物学机制的研究 在氧毒性中发生的表面活性剂相关变化，以及 确定治疗干预的有效性， 细胞水平。 实验使用了最先进的技术， 包括激光流式细胞术，以研究体内氧的影响 暴露对II型肺细胞代谢功能的影响。 这些研究将 然后结合实验来确定细胞的功效， 定向抗氧化治疗。

The applicant is an assistant professor of Gyn/Ob, Pediatrics, and Pharmacology with considerable experience in the biology of the pulmonary surfactant system and in surfactant replacement therapy. The applicant's long term career objective is to develop a multidisciplinary research program which will allow him to combine interests in basic pulmonary cell biology with experimental therapeutic approaches to the treatment of acute lung disease. The candidate's more immediate goals are to expand the current cell biology expertise of his laboratory, and to gain more hands-on experience in the area of molecular biology. The RCDA will allow the applicant to decrease his current teaching and administrative responsibilities in order to spend full time in the laboratory and achieve these goals. The applicant's Institution is supportive of his goals and agree with the candidate's perception of how to achieve them. There are a number of NIH-funded senior faculty at the State University of New York with interests in pulmonary research who collaborate with the applicant and have formed a Cardio-Pulmonary Research Group to facilitate communication. In addition, the University is proceeding with a new research building, slated for completion by 1992-93, which will provide extensive space for groups of investigators with common interest. Finally, the applicant has extensive collaborations with the University of Rochester and has been invited to spend time in the laboratory of Dr. Stuart Horowitz there in order to gain training in molecular biology. The research proposed for this award period involves studies of the cell biological mechanisms responsible for surfactant-related changes which occur in oxygen toxicity, and determination of the efficacy of therapeutic interventions directed at the cellular level. The experiments use state of the art techniques, including laser flow cytometry, to study the effects of in vivo oxygen exposure on type II pneumocyte metabolic function. These studies will then be combined with experiments to determine the efficacy of cell directed anti-oxidant therapy.