RECONSTITUTION OF A NEURONAL TRANSMITTER RELEASE SYSTEM

这个项目的目标是提高我们对发射机的了解。 神经释放过程及其对突触前事件的调控 细胞。这种方法需要重建神经递质。 非洲爪哇卵母细胞释放系统的研究。这些卵母细胞可以 显微注射了各种各样的材料，重要的是，已经 显示能翻译外源提供的信使RNA。重建 发布过程包括三个阶段。首先，卵母细胞将被 注射来自鱼雷或鱼雷的电动神经元的信使RNA 纳尔辛。这一步可能是赋予卵母细胞成分的关键。 需要支持递质的胞吐放电。卵母细胞会 然后注射从电浆中分离出来的突触小泡 鱼雷或水母的组织。最后，卵母细胞将被刺激 以电或化学方法确定是否诱发释放 可以检测到递质(乙酰胆碱)。这件事成功了 重建工作将有助于对 参与递质分泌的分子成分。由此产生的 观察可能为研究生物化学相关性开辟新的途径。 突触可塑性。此外，广泛的神经缺陷(例如， 痴呆症、帕金森氏症、智力低下)可能需要仔细检查 从一个新的角度来看。

The goal of this project is to improve our understanding of the transmitter release process and the means of regulating presynaptic events in nerve cells. The approach entails the reconstitution of a neuronal transmitter release system using the oocytes of Xenopus laevis. These oocytes can be microinjected with a wide range of materials, and importantly, have been shown to translate exogenously supplied messenger RNA. The reconstruction of the release process involves three phases. First, oocytes will be injected with messenger RNA from electromotor neurons of Torpedo or Narcine. This step may be crucial for endowing oocytes with components needed to support the exocytotic discharge of transmitter. The oocyte will then be injected with synaptic vesicles isolated from the electroplax tissue of Torpedo or Narcine. Finally, the oocytes will be stimulated electrically or chemically to determine whether evoked release of transmitter (acetylcholine) can be detected. Success of this reconstitution effort will facilitate a detailed examination of the molecular components involved in transmitter secretion. The resulting observations may open new avenues to the study of biochemical correlates of synaptic plasticity. Moreover, a wide range of neurological deficits (eg., dementia, Parkinsonism, mental retardation) might be amenable to scrutiny in a new light.