DEVELOPMENT REGULATION OF HIV GENE EXPRESSION & LATENCY

先天性艾滋病和成人艾滋病之间的一个关键区别是 先天性心脏病的潜伏期缩短或消失 疾病。与先天性艾滋病相比，先天性艾滋病潜伏期缩短或消失 成人艾滋病。作为新生儿免疫反应和淋巴细胞 与成人有质的不同，这一提议的假设是 新生儿淋巴细胞含有改变HIV基因的因子 这样才不会出现延迟。以类似的方式， 被认为是DNA病毒重叠感染或免疫刺激 来结束成年人的潜伏期。计划中的研究将比较成人和 脐带血淋巴细胞介导的HIV转录、DNA结合 蛋白质，并鉴定与脐带血相互作用的HIV序列 核因素。目标是分离和克隆脐带血 HIV表达改变的相关因素及其比较 以及其他被认为影响艾滋病毒表达的因素。今年5月 提供对潜伏期的洞察并建议新的治疗方法。 初步结果显示，脐带和脐带之间存在显著差异。 成体核抽提物与LTR片段的结合 序列。

A key difference between congential and adult AIDS is the reduced or absent latency period observed in the congenital disease. Congenital AIDS has reduced or absent latency compared to adult AIDS. As newborn immune responses and lymphocytes differ qualitatively from adult's, this proposal's hypothesis is that newborn lymphocytes contain factors that alter HIV gene expression so that latency can not occur. In an analogous fashion, superinfection with DNA virus or immune stimulation are thought to end latency in adults. Planned studies will compare adult and cord blood lymphocyte-mediated HIV transcription, DNA binding proteins, and identify HIV sequences that interact with cord blood nuclear factors. The goal is to isolate and clone cord blood factors implicated in altered HIV expression and compare them with other factors thought to affect HIV expression. This may provide insight into latency and suggest new therapies. Preliminary results show substantial differences between cord and adult nuclear extract bonding to LTR fragments with regulatory sequences.