HEPATIC METABOLISM OF ALCOHOL BY GSH TRANSFERASE

拟议的工作将集中在发展一个分子的理解， 肝脏中的非氧化性酒精代谢，受 谷胱甘肽转移酶。 具体目标包括隔离和 这些酶的纯化和描述 在肝脏中对酒精的代谢很活跃。 研究还将包括 通过该途径代谢的酒精量的定量。 研究将涉及致癌物质和酒精的共同代谢， 谷胱甘肽转移酶和分子遗传学研究将检查 乙醇诱导谷胱甘肽转移酶的机制。 这项工作代表了兰格博士的一个新方向， 与此同时，研究致癌物质和乙醇代谢之间的关系， 环境影响改变酒精的分子解释 肝脏内的代谢率。

The proposed work will focus on developing a molecular understanding of nonoxidative alcohol metabolism in the liver, as effected by the glutathione transferases. Specific goals include the isolation and purification of these enzymes and description of which isoenzymes are active in metabolizing alcohol in the liver. Studies will also include the quantitation of the amount of alcohol metabolized through this pathway. Studies will relate the co-metabolism of carcinogens and alcohol by the glutathione transferases and molecular genetic studies will examine the mechanism of the alcohol-induced induction of the glutathione transferases. This work represents a new direction for Dr. Lange and potentially can relate carcinogen and ethanol metabolism and, at the same time, examine molecular explanations for environmental influences on altering alcohol metabolic rates within the liver.