DEVELOPMENT REGULATION OF HIV GENE EXPRESSION & LATENCY

先天性艾滋病和成人艾滋病之间的一个关键区别是 在先天性脊髓损伤中观察到的潜伏期缩短或缺失 疾病 先天性艾滋病的潜伏期减少或不存在， 成人艾滋病 新生儿的免疫反应和淋巴细胞 与成人的有质的不同，这个建议的假设是， 新生儿淋巴细胞含有改变HIV基因的因子 表达式，以便不会发生延迟。 以类似的方式， 认为DNA病毒或免疫刺激双重感染 to end结束latency潜伏in adults成人. 计划中的研究将比较成人和 脐血淋巴细胞介导的HIV转录，DNA结合 蛋白质，并确定与脐带血相互作用的HIV序列 核因素。 目的是分离和克隆脐带血 与HIV表达改变有关的因素，并将它们 与其他被认为影响艾滋病毒表达的因素。 这可能 提供对潜伏期的深入了解并提出新的治疗方法。 初步结果显示，脐带血和 成人核提取物与LTR片段结合， 序列的

A key difference between congential and adult AIDS is the reduced or absent latency period observed in the congenital disease. Congenital AIDS has reduced or absent latency compared to adult AIDS. As newborn immune responses and lymphocytes differ qualitatively from adult's, this proposal's hypothesis is that newborn lymphocytes contain factors that alter HIV gene expression so that latency can not occur. In an analogous fashion, superinfection with DNA virus or immune stimulation are thought to end latency in adults. Planned studies will compare adult and cord blood lymphocyte-mediated HIV transcription, DNA binding proteins, and identify HIV sequences that interact with cord blood nuclear factors. The goal is to isolate and clone cord blood factors implicated in altered HIV expression and compare them with other factors thought to affect HIV expression. This may provide insight into latency and suggest new therapies. Preliminary results show substantial differences between cord and adult nuclear extract bonding to LTR fragments with regulatory sequences.