CHARACTERIZATION OF PLACENTAL PHOSPHOLIPASE A2 ISOZYMES

磷脂酶A2（PLA 2）是一种普遍存在的蛋白质，存在于每一个 细胞在哪里寻找。这种酶催化水解 磷脂的sn-2-酰基位置。磷脂酶A2是一种 重要的酶。水解时释放的游离脂肪酸 的磷脂进一步代谢成各种生物制剂 如白细胞三素和白藜芦醇。PLA 2的活性已被 在各种炎症中实施，例如哮喘和关节炎。 关于这些酶如何起作用的化学信息将是 可用于药理学和医学领域。这些信息 可以帮助设计特定的药物。 对牛胰腺PLA 2的催化机理进行了推测 对钙依赖酶的作用然而，动力学分析的 人钙依赖性和钙非依赖性PLA 2还没有被 报道 我们建议研究和比较底物特异性和结构 钙依赖型与非钙依赖型的相关性 PLA 2来自人胎盘此外，我们计划确定 对这些磷脂酶的抑制剂。我们的目标是更好地 表征酶，这将导致未来的研究， 更好地定义其生理功能。

Phospholipase A2 (PLA2) is an ubiquitous protein that is found in every cell where it has been sought. This enzyme catalyzes the hydrolysis of the sn-2- acyl position of phospholipids. Phospholipase A2 is an important enzyme. The free fatty acid which is released upon hydrolysis of the phospholipid is further metabolized into various biological agents such as leukotriences and prostaglandins. The activity of PLA2 has been implemented in various inflammatory as for example, asthma and arthritis. Information on the chemistry of how these enzymes function would be useful for both the pharmacological and medical fields. This information could aid in the design of specific drugs. The catalytic mechanism for the bovine pancreas PLA 2 has been postulated for the calcium dependent enzyme. However, the kinetic analysis of the human calcium dependent and calcium- independent PLA2's has not been reported. We propose to study and compare the substrate specificity and structural relatedness of the calcium - dependent and the calcium- independent PLA2's from human placenta. In addition, we plan to determine the effect of selected inhibitors on these phospholipases. Our aim is to better characterize the enzyme which would lead to future studies so as to better define their physiological function.