CHARACTERIZATION OF PLACENTAL PHOSPHOLIPASE A2 ISOZYMES

磷脂酶A2(PLA2)是一种普遍存在的蛋白质，存在于 被寻找的单元格。这种酶催化水解酶 磷脂的sn-2-酰基位置。磷脂酶A2是一种 重要的酵素。水解物中释放的游离脂肪酸 磷脂被进一步代谢成各种生物制剂 例如白质营养素和前列腺素。磷脂酶A2的活性一直是 用于各种炎症性疾病，如哮喘和关节炎。 关于这些酶如何发挥作用的化学信息 对药理学和医学领域都有用。此信息 可以帮助设计特定的药物。 对牛胰腺磷脂酶A2的催化机理进行了推测 对于钙依赖的酶。然而，对这一现象的动力学分析 人钙依赖和非钙非依赖性磷脂酶A_2‘S 据报道。 我们建议研究和比较底物的特异性和结构 钙依赖与非钙依赖的相关性 PLA2‘S来自人胎盘。此外，我们计划确定影响 这些磷脂酶的选定抑制剂。我们的目标是更好地 描述这种酶的特性，这将导致未来的研究，以便 更好地定义它们的生理功能。

Phospholipase A2 (PLA2) is an ubiquitous protein that is found in every cell where it has been sought. This enzyme catalyzes the hydrolysis of the sn-2- acyl position of phospholipids. Phospholipase A2 is an important enzyme. The free fatty acid which is released upon hydrolysis of the phospholipid is further metabolized into various biological agents such as leukotriences and prostaglandins. The activity of PLA2 has been implemented in various inflammatory as for example, asthma and arthritis. Information on the chemistry of how these enzymes function would be useful for both the pharmacological and medical fields. This information could aid in the design of specific drugs. The catalytic mechanism for the bovine pancreas PLA 2 has been postulated for the calcium dependent enzyme. However, the kinetic analysis of the human calcium dependent and calcium- independent PLA2's has not been reported. We propose to study and compare the substrate specificity and structural relatedness of the calcium - dependent and the calcium- independent PLA2's from human placenta. In addition, we plan to determine the effect of selected inhibitors on these phospholipases. Our aim is to better characterize the enzyme which would lead to future studies so as to better define their physiological function.