GENETIC MARKERS FOR PREDICTING PROGNOSIS OF BREAST CANCERS

这些研究的目标是开发新的遗传标记来预测 乳腺癌的预后。它将：1)利用消减杂交 寻找新的基因；2)进一步评估等位基因的意义 染色体1q23-32的丢失，首次由该项目描述，与长的- 在这个基因座克隆一个假定的抑制基因的学期目标。会的 还可以测量随机遗传基因座和/或 放大以检验假设，特定的 导致侵袭性肿瘤行为的基因改变直接 与该肿瘤的总遗传畸变率成正比。 它将检查乳腺癌以寻找异常甲基化的证据 确定甲基化异常是否与等位基因相关 不稳定。对于选定的乳腺癌样本，正常细胞(或 血液或皮肤)将可从患者和父母双方获得。在……里面 在这些情况下，将进行研究，以评估母亲的作用 通过确定母亲的等位基因是否为 优先输掉。

The goal of these studies is to develop new genetic markers for predicting prognosis of breast cancer. It will: 1) utilize subtractive hybridization to find new genes; and 2) further evaluate the significance of an allelic loss at chromosome 1q23-32, first described by this project, with the long- term goal of cloning a putative suppressor gene at this locus. It will also measure the frequency of allelic losses at random genetic loci and/or amplifications to test the hypothesis that the likelihood of a specific genetic alteration conferring aggressive tumor behavior is directly proportional to the overall frequency of genetic aberrations in that tumor. It will examine breast cancers for evidence of aberrant methylation to determine whether abnormal methylation is associated with allelic instability. For selected breast cancer specimens, normal cells (either blood or skin) will be available from the patient and both parents. In these cases, studies will be performed to evaluate the role of maternal imprinting in breast cancer by determining whether the maternal allele is preferentially lost.