REGENERATION & FUNCTIONAL RECOVERY IN NEURAL TISSUE

我们的目标是增加对支配神经的因素的基础知识 纤维生长，在目标组织中形成适当的连接，以及 这些连接在实验操作后的修改。 个别项目试图调查：a)如果雪旺细胞或 周围神经成纤维细胞为神经元提供营养支持(在 组织培养)，如果这些细胞排列成“桥”，培养出轴突 (动物)受损脊髓的再生，b)轴突的成功 正常和修饰的“底物”和In的生长和连接形成 胎儿和新生儿中枢神经系统束中常见靶点的缺失 动物，c)突触活动的发育与FINE的相关性 突触形成过程中生长锥和靶细胞的结构变化， D)控制特定蛋白质合成的信号，这些蛋白质可能发挥作用 在轴突再生中的关键作用，e)培养系统的发展 确定神经胶质细胞在正常来源神经细胞迁移中的作用 和遗传异常动物，f)的药理学基础 视觉皮质的可塑性，g)生理和解剖学 婴儿期或成年期受损的动物之间的差异 前者表现出行为可塑性，以及h)是否可以检测到学习 在小脑的细胞水平上，从而作为一种模型 研究行为的自适应控制。涉案的九名调查人员 带来不同的实验范例和技术方面的专业知识。这个 这项提议的目的是寻求资金支持共享设施 (计算机、电子显微镜和动物支持)和刺激 互动和协作，以促进实现以下目标的进展 更好地理解神经元生长和相互作用的基本机制。 我们需要关于轴突再生和有用的机制的新知识 如果我们要成功地干预以促进再生，就会发生可塑性 在受折磨的人类患者的中枢神经系统中。

Our goal is to increase fundamental knowledge of factors that govern nerve fiber growth, formation of appropriate connections in target tissues, and modifications of these connections following experimental manipulation. The individual projects seek to investigate: a) if Schwann cells or fibroblasts from peripheral nerve provide trophic support for neurons (in tissue culture) and if these cells, aligned in "bridges", foster axonal regrowth in damaged spinal cord (in the animal), b) the success of axonal growth and connection formation of normal and modified "substrata" and in the absence of the usual targets in CNS tracts in fetal and neonatal animals, c) the correlation of developing synaptic activity with fine structural changes in growth cone and target cell during synapse formation, d) the signals that govern synthesis of specific proteins that may play a key role in axonal regeneratio, e) the development of a culture system to determine the role of glia in migration of nerve cells derived from normal and genetically abnormal animals, f) the pharmacological basis for plasticity in the visual cortex, g) physiological and anatomical differences between animals lesioned in infancy or adulthood with only the former exhibiting behavioral plasticity, and h) if learning can be detected at the cellular level in the cerebellum and thus serve as a model for studying adaptive control of behavior. The nine investigators involved bring expertise in diverse experimental paradigms and techniques. The purpose of this proposal is to seek funds to support shared facilities (computer, electron micro scopy, and animal support) and to stimulate interaction and collaboration to facilitate progress toward the goal of better understanding basic mechanisms of neuronal growth and interactions. We need new knowledge of mechanisms by which axonal regeneration and useful plasticity occur if we are to successfully intervene to prompt regeneration in the CNS of afflicted human patients.