ROLE OF ETHANOL IN FREE RADICAL GENERATION

酒精可能会导致膜结构和功能恶化 这是由于自由基形成的增强。 的 这项提案的总体目标是调查酒精如何 摄食可通过这种机制引起膜改变。 具体而言，实验将被设计为（1）研究 乙醇诱导的自由基形成机制，（2）试验 假设自由基可能发挥：主要作用， （3）评价慢性酒精性肝损伤的影响 酒精摄入对特定膜功能和结构的影响，（4） 评估肝脏和血细胞中的机体保护系统 膜对抗自由基的毒性作用和（5） 研究饮食中抗氧化剂的作用，如维生素 E，防止酒精的过氧化作用。 电子自旋共振谱技术 将采用自由基的检测和识别。 由于生理和行为的相似性辛克莱（S- l）微型猪到人类，这些动物将用于 研究急性和慢性酒精摄入对血液的影响 细胞和肝膜。 这些猪特别适合 酒精研究，因为他们是众所周知的消耗乙醇 自愿的 大多数研究建议将利用红细胞 血影和亚细胞膜。 膜脂组成，特别是多不饱和脂肪酸 磷脂和一些膜结合活性， 因为ATP酶将在急性和慢性乙醇后检查， 局 酶的活性参与身体的 抗活性氧的保护系统，如超氧化物 将对歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶进行评价 also. 预计通过此次调查， 可以获得关于膜的机制的信息， 由于酒精摄入而恶化。 此外，信息 将获得关于膳食补充剂的有效性 维生素E和C在缓解一些变化，由于 酗酒

Alcohol may cause deterioration of membrane structure and function due to the enhancement of free radical formation. The overall objective of this proposal is to investigate how alcohol ingestion may cause membrane alteration through this mechanism. Specifically, experiments will' be designed to (1) study the mechanism of free radical formation as induced by ethanol, (2) test the hypothesis that free radicals may play a: major role in alcohol-induced liver injury, (3) evaluate the effects of chronic alcohol ingestion on specific membrane function and structure, (4) evaluate the body's protective system in liver and blood cell membranes against the toxic effects of free radicals and (5) investigate the effects of dietary antioxidants, such as vitamin E, in protecting against the peroxidation effects of alcohol. The technique of electron spin resonance spectroscopy for the detection and identification of free radicals will be employed. Due to the physiological and behavioral similarity of Sinclair (S- l) miniature swine to humans, these animals will be used for studying the effect of acute and chronic ethanol ingestion on blood cells and liver membranes. These swine are especially suitable for alcohol studies because they are known to consume ethanol voluntarily. Most studies proposed will utilize the red blood cell ghost and the subcellular membranes from liver biopsy samples. Membrane lipid composition, especially polyunsaturated fatty acid profile of phospholipids, and some membrane-bound activities such as ATPases will be examined after acute and chronic ethanol administration. Activity of enzymes involved in the body's protective system against active oxygen species, such as superoxide dismutase, catalase and gluthatione peroxidase will be evaluated also. It is anticipated that through this investigation, useful information may be obtained regarding the mechanism of membrane deterioration due to alcohol ingestion. ln addition, information will be obtained regarding the effectiveness of dietary supplement of vitamins E and C in alleviating some of the changes due to alcohol abuse.