FILAMENTOUS PHAGE PHYSIOLOGY

我计划研究丝状生物的膜相关组装 一组感染携带F生育力的细胞的病毒 因子 我最初的方法有两个部分：开发简单的检测方法， 用于病毒组装阶段，以及病毒的构建和分离 以及影响组装过程的宿主细胞突变体， 受影响的特定步骤可以用阶段特异性测定来鉴定。 这项工作应能更清楚地了解各组成部分 参与装配，并为深入研究该过程奠定基础 体外 如果组装传染性病毒是可行的 这可能是克隆系统的基础 非常大的外来DNA片段 我还计划研究其他方法， 丝状噬菌体生理学知识可以用来设计新的 操纵和分析克隆DNA的方法。

I plan to investigate the membrane-associated assembly of the filamentous phages, a group of viruses that infect cells carrying the F fertility factor. My initial approach has two parts: development of simple assays for the stages of virus assembly, and construction and isolation of viral and host-cell mutants that affect the assembly process, so that the specific step affected can be identified with the stage-specific assays. This work should result in a much clearer picture of the components involved in assembly, and set the stage for a deeper study of the process in vitro. If it turns out to be feasible to assemble infectious viruses from naked DNA in vitro, this might be the basis of a system for cloning very large pieces of foreign DNA. I also plan to study other ways in which knowledge of filamentous phage physiology might be expoited to devise new ways of manipulating and analyzing cloned DNA.