THE ROLE OF AEROMONAS HYDROPHILA VIRULENCE FACTORS

这项拟议研究的长期目标是确定 嗜水气单胞菌毒力因子在疾病发病机制中的作用 天哪。目前在相关文献中报道的毒力因子 对嗜水气单胞菌的定义尚不明确。A.嗜水线虫，成员 属于弧菌科，被认为是一种重要的人类病原体。这 微生物是引起各种疾病的元凶，包括 急性细菌性腹泻、败血症、脑膜炎、心内膜炎、角膜 溃疡、腹膜炎和伤口感染。这些疾病发生在 具有免疫能力和免疫功能受损的个人。嗜水气单胞菌产生 以及拥有一些可能有助于总体 生物体的毒性。我们发现嗜水气单胞菌、分离物 在酪氨酸-酵母提取物(CyE)肉汤中生长的SSU可以产生许多 在某些嗜水气单胞菌中起作用的相关毒素 疾病。我们将其中一种毒素命名为霍乱毒素交叉反应。 (CTC)溶细胞素“，因为它能与霍乱抗毒素和 引起兔肠道内液体蓄积以及其 中国仓鼠卵巢细胞和兔红细胞的破坏。我们的 实验室已将细胞毒性肠毒素CTC-溶血素提纯为 同源性和氨基酸序列分析显示，前25个 N-末端的氨基酸与溶气素的氨基酸相同 霍华德和巴克利报道。此外，我们已经提纯到 同源的一种细胞毒性肠毒素，称为“非CTC肠毒素” 因为它不与霍乱抗毒素反应，不溶于细胞 但可引起兔肠内液体蓄积 循环。我们未来的目标将是进一步描述 这些毒素的抗原性、生物学和生化性质；确定 与之相关的生物活动的作用机制 CTC-溶细胞素和非CTC肠毒素；发现遗传型(S) 气单胞菌CTC-溶血素和非CTC合成的信息编码 肠毒素。关于CTC溶细胞素和非CTC的拟议研究 肠毒素和其他毒力因子可能有助于阐明 嗜水气单胞菌引起人类疾病的方式。

The long term objective of this proposed research is to determine the role of Aeromonas hydrophila virulence factors in the pathogenesis of disease in man. The virulence factors presently reported in the literature associated with A. hydrophila have not been clearly defined. A. hydrophila, a member of the family Vibrionaceae,is considered a significant human pathogen. This microorganism is responsible for causing a variety of diseases including acute bacterial diarrhea, septicemia, meningitis, endocarditis, corneal ulcers, peritonitis, and wound infections. These diseases have occurred in immunocompetent and immunocompromised individuals. A. hydrophila produce as well as possess a number of factors which may contribute to the overall virulence of the organism. We have discovered that A. hydrophila, isolate SSU, grown in Casamino acid-yeast extract (CYE) broth, can produce a number of interrelated toxins that play a role in some A. hydrophila mediated diseases. We have termed one of these toxins "cholera-toxin crossreactive (CTC) cytolysin" because of its ability to react with cholera antitoxin and cause fluid accumulation in rabbit intestinal loops as well as its destruction of Chinese hamster ovary cells and rabbit red blood cells. Our laboratory has purified CTC-cytolysin, a cytotoxic enterotoxin, to homogeneity and an amino acid sequence analysis revealed that the first 25 amino acids from the N-terminal end were identical to that of aerolysin reported by Howard and Buckley. In addition, we have purified to homogeneity a cytotonic enterotoxin and it was termed "non-CTC enterotoxin" because it does not react with cholera antitoxin and is free of cytolytic activity but is capable of causing fluid accumulation in rabbit intestinal loops. Our goals for the future will be to further characterize the antigenic, biological, and biochemical nature of these toxins; to determine the mechanism of action of the biological activities associated with CTC-cytolysin and non-CTC enterotoxin; to discover the type(s) of genetic information coding for the synthesis of Aeromonas CTC-cytolysin and non-CTC enteroxin. The proposed research involving CTC cytolysin and non-CTC enterotoxin as well as other virulence factors may aid in elucidating the means by which A. hydrophila cause human disease.