SUPPRESSOR T CELLS IN HAPTEN SPECIFIC IMMUNITY

这笔赠款的目的是分析H-2与 编码基因产物和类VH基因产物 抑制T细胞(Ts)途径。I-J行列式之间的关系 一阶ts细胞上的独特型成分将进行一系列的研究。 以检查I-J和VH结构的优先分类 它们存在于抑制分子(TSF)上。H-2的遗传基础 发生在二级(TS2)和三级(TS3)之间的限制 细胞将通过评估遗传限制的诱导来进行检查 用于生成TS3的信号。此外，通过在 并检测产生的TsF2的能力 在F1杂交或其他近交系中的功能，我们将确定H-2 控制TS2激活和功能的基因座。而且，这个特殊的角色 I-J+抗原提呈细胞(APC)的数量将使用体外 细胞毒性实验，我们可以选择性地操纵APC的活性。我们 还将描述两个分子的功能和结构特性 长期杂交瘤。我们将在体外和体内评估功能 在每一级的提纯和表征中。最后使用一个 分子生物学的方法，我们将评估其活性和结构 由从这些细胞中获得的纯化和分离的mRNA翻译的蛋白质 杂交瘤。因此，这些研究的主要目的是分析 Ts细胞及其产物在半抗原特异性免疫中的免疫生物学研究。

The objective of this grant is to analyze the relationship between H-2 encoded gene products and VH like gene products which are important in suppressor T cell (Ts) pathways. The relationship between I-J determinants and idiotypic elements on first order Ts cells will be examined in a series of F1 hybrids to examine the preferential assortment I-J and VH structures which are present on suppressor molecules (TsF). The genetic basis of H-2 restriction which occurs between second order (Ts2) and third order (Ts3) cells will be examined by evaluating the genetically restricted inductive signals for Ts3 generation. Moreover, by generating Ts2 and TsF2 in a panel of inbred strains and examining the ability of such generated TsF2 to function in F1 hybrids or other inbred strains, we will determine the H-2 loci which govern Ts2 activation and function. Moreover, the special role of I-J+ antigen presenting cells (APC) will be deduced using an in vitro cytotoxicity assay in which we can selectively manipulate APC activity. We will also characterize the functional and structural properties of two long-term Ts hybridomas. We will evaluate in ivitro and in vivo function at each level of pruification and characterization. Finally using a molecular biological approach, we will evaluate the activity and structure of proteins translated by purified and isolated mRNA obtained from these hybridomas. The major thrust of these studies is therefore to analyze the immunobiology of Ts cells and their products in hapten specific immunity.