REGULATION OF EXPRESSION OF ADHERENCE FACTORS

建立传染性腹泻病的第一步是 入侵的病原菌附着在宿主上 组织. 在产肠毒素大肠杆菌（ETEC）的情况下， 这是由定植因子抗原介导的， 蛋白质表面结构的主要亚基， 细菌（皮利或菌毛）。 CFA/I、CFA/II和E8775已被 迄今为止在人类ETEC菌株中发现。 CFA/II由以下组成 三种独立的抗原，称为CS 1、CS2和CS3。 这些抗原 质粒编码，但它们的表达受宿主调控， CS 1和CS2的表达是互斥的。 我们建议 将分子技术应用于CFA/II抗原的研究， 关于宿主对质粒编码功能的调节 这项工作 应该能让我们理解 CS2皮利的遗传控制和功能。 我们希望 收集的信息将导致一个战略的发展， 预防人类ETEC菌株的疫苗，或可能预防 粘附素类似物治疗。

The first step in establishment of infectious diarrheal disease is the attachment of the invading pathogenic bacteria to the host tissue. In the case of enterotoxigenic Escherichia coli (ETEC), this is mediated by colonization factor antigens which constitute the major subunit of proteinaceous surface structures on the bacteria (pili or fimbriae). CFA/I, CFA/II and E8775 have been identified so far in human ETEC strains. CFA/II is composed of three separate antigens, called CSl, 2, and 3. These antigens are plasmid encoded, but their expression is regulated by the host and expression of CSl and CS2 is mutually exclusive. We propose to apply molecular techniques to the study of CFA/II antigens to learn about host regulation of plasmid-encoded functions. This work should lead to the understanding of the structure, assembly, genetic control and function of CS2 pili. We hope that the information gleaned will lead to a strategy for the development of a vaccine to protect against human ETEC strains or possibly to an adhesin analog therapy.