HUMAN RHEUMATOID FACTOR CROSS-IDIOTYPES

三种人单克隆类风湿因子的发生 (mRF)多克隆间的交叉独特型（XIds）WA、PO和BLA 各种疾病中的类风湿因子和免疫球蛋白 将使用小鼠单克隆抗体进行研究， 热不稳定构象重-轻链的特异性 XId抗原，其是由免疫球蛋白识别的主要决定簇。 定义mRF XIds的原始多克隆抗血清。 在B细胞库中mRF XIDS的发生也将被评估。 访问。 重链和轻链在形成XId中的作用 抗原和确定mRF的抗原特异性 三组中的哪一组将由组合的轻重来决定 链重组。 对PO XId的研究将继续进行 初步数据表明，这个XId决定因素，在 与WA和BLA XIDS相反，与 抗原结合位点。 将特别强调学习 BLA XId的发生率较高， 类风湿性关节炎中的多克隆RF。 RF轴承 这种XId具有独特特异性，因为它们与DNA反应， 组蛋白2A-2B，除IgG外的抗原。 的氨基酸 的轻链和重链的可变区的序列 这一组的原型单克隆和多克隆RF将被 决心寻找不仅是结构上的见解， 独特型-抗原特异性关系，但也有限制， 可能存在于自身抗体的产生中。 的发生 各种mRF互补决定区（CDR）抗原 多克隆类风湿因子，其他多克隆自身抗体 和正常免疫球蛋白使用抗血清提高到合成 将测定CDR肽以检验以下假设： 某些CDR抗原选择性地与 自身抗体

The occurrence of the three human monoclonal rheumatoid factor (mRF) cross idiotypes (XIds) WA, PO and BLA among polyclonal rheumatoid factors in various diseases and among immunogobulins in normals will be studied using mouse monoclonal antibodies with specifities for the heat labile conformational heavy-light chain XId antigens that are the main determinants recognized by the original polyclonal antisera which defined the mRF XIds. Occurrence of the mRF XIds in the B cell repertoire will also be accessed. The role of heavy and light chains in forming the XId antigens and in determining the antigen specificities of the mRFs of the three groups will be determined by assorted heavy-light chain recombinations. Studies on the PO XId will persue preliminary data which suggests that this XId determinant, in contrast to the WA and BLA XIds, is not associated with the antigen combining site. Special emphasis will be placed on study of the BLA XId which has been shown to occur in high incidence among polyclonal RFs in rheumatoid arthritis. The RFs bearing this XId have unique specificity in that they react with DNA- histone 2A-2B, antigen in addition to IgG. The amino acid sequence of the variable regions of the light and heavy chains of the prototype monoclonal and polyclonal RFs of this group will be determined in search of insights not only to the structural- idiotype-antigen specificity relationships but also restrictions that may be present in autoantibody production. The occurrence of various mRF complementary determining region (CDR) antigens on polyclonal rheumatoid factors, other polyclonal autoantibodies and normal immunoglobulins using antisera raised to synthetic CDR peptides will be determined to test the hypothesis that certain CDR antigens are selectively associated with autoantibodies.