ANALYSIS OF BORRELIA BURGDORFERI MOTILITY

莱姆病是美国最普遍的节肢动物传播疾病 States. 它与关节炎、心脏炎和神经系统疾病有关。 损害 这种疾病的发病率正在上升，在一些 社区是一个主要的健康问题。 伯氏疏螺旋体是 莱姆病的病原体。 其生物学的基本方面，让 更不用说它是如何致病的了 B的一个特点。burgdorferi是其快速运动性， 在粘性环境中游泳的能力。 动力可能与此有关 螺旋体侵入并随后损害组织和器官， 大脑脑膜和眼睛 因为我们对这种生物 游泳，该补助金拟为B奠定基础。burgdorferi 能动性 所采用的方法是多方面的，包括遗传， 显微电影、超微结构和生物化学方法。 诱变剂诱导和自发发生的B运动突变体。 将通过标准程序分离伯氏菌。 主要做法是 分离不能在琼脂糖平板上扩散的菌落。 回复突变体 运动性将通过平板接种突变体获得，并挑选 长时间培养后突变菌落的扩散生长。 自由游动和拴系的野生型、突变体和回复突变体细胞将被 使用视频显微镜进行分析，频闪照明多个 曝光摄影和高速电影摄影。 几个参数 将进行监测，并将借助 电脑 初步的多重曝光摄影结果表明， 生物体在游泳过程中改变其形状：一个平移，两个平移。 观察到非翻译形式。 基于周质的假说 鞭毛的旋转是这些形式的原因。 野生型、突变体和回复突变体的结构将通过 暗场光学显微镜、透射和扫描电子显微镜 显微镜 来自特定突变体的周质鞭毛将是 具有形态学和生物化学特征。 本地化具体 突变，选择突变体的周质鞭毛丝基因， 回复突变体和野生型将被克隆和测序。 结果 获得的数据应能够得出有关的模型和结论， B的机制。伯氏运动

Lyme disease is the most prevalent arthropod borne disease in the United States. It is associated with arthritis, carditis, and neurological damage. The incidence of this disease is on the increase, and in some communities it is a major health problem. Borrelia burgdorferi is the causative agent of Lyme disease. Fundamental aspects of its biology, let alone how it causes disease, are yet to be discovered. One special characteristic of B. burgdorferi is its rapid motility and capacity to swim in viscous environments. Motility may be involved in this spirochete's invading and subsequently damaging tissues and organs such as brain, meninges, and eye. Because little is known about how this organism swims, this grant proposes to lay a foundation concerning B. burgdorferi motility. The approach used is multifaceted and includes genetic, microcinematographic, ultrastructural, and biochemical methods. Both mutagen induced and spontaneously occurring motility mutants of B. burgdorferi will be isolated by standard procedures. The main approach is isolating colonies which fail to spread on agarose plates. Revertants to motility will be obtained by plating out the mutants, and picking areas of spreading growth arising from mutant colonies after prolonged incubation. Free swimming and tethered wild-type, mutant, and revertant cells will be analyzed using videomicroscopy, stroboscopically illuminated multiple exposure photography, and high speed cinematography. Several parameters will be monitored, and the results will be analyzed with the aid of a computer. Preliminary multiple exposure photography results indicate that the organism changes its shape during swimming: one translational and two non-translational forms are observed. An hypothesis based on periplasmic flagella rotation is presented which accounts for these forms. The structure of the wild-type, mutants, and revertants will be analyzed by dark-field light microscopy, and transmission and scanning electron microscopy. The periplasma flagella from specific mutants will be morphologically and biochemically characterized. To localize specific mutations, the periplasmaic flagella filament gene from select mutants, revertants, and the wild-type will be cloned and sequenced. The results obtained should enable models and conclusions be drawn concerning mechanisms of B. burgdorferi motility.