IMMUNOGLOBULIN-RELATED PROTEINS IN INSECT HEMOLYMPH

昆虫能够通过防御行动对微生物感染作出反应。 血细胞(血细胞)和抗菌蛋白的合成 被胖乎乎的身体。昆虫识别非我的机制 细胞是未知的。一种细菌诱导的M(R)=48000蛋白的分离 从烟草天牛的血淋巴中发现 通过对其cDNA序列的分析，确定其为免疫球蛋白的一员 基因超家族。这项建议的长期目标是 了解与免疫球蛋白相关的蛋白质在昆虫中的作用 免疫系统。其具体目的是：1)分离和测序 和免疫球蛋白相关血淋巴成员的基因组克隆 Manduca中的蛋白(IRHP)家族：2)IRHP基因表达的调控； 3)IRHP功能鉴定；4)免疫球蛋白相关研究 在血细胞中表达的蛋白质；5)果蝇IRHP的研究。这个 对昆虫免疫球蛋白相关蛋白的研究可能会提供信息 与理解昆虫作为人和动物的媒介有关 并有助于了解人类疾病的进化过程 免疫球蛋白基因超家族。

Insects are able to respond to microbial infections by defensive actions of the hemocytes (blood cells) and by synthesis of antibacterial proteins by the fat body. The mechanisms by which nonself is recognized by insect cells are unknown. A bacteria-induced protein of M(r) = 48,000 isolated from the hemolymph of the tobacco hornworm, Manduca sexta, has been shown by analysis of its cDNA sequence to be a member of the immunoglobulin gene superfamily. The long-term objective of this proposal is to understand the role of proteins related to immunoglobulins in the insect immune system. Specific aims are: 1) Isolation and sequencing of cDNA and genomic clones for members of the immunoglobulin-related hemolymph protein (IRHP) family in Manduca; 2) Regulation of IRHP gene expression; 3) Identification of IRHP functions; 4) Studies of immunoglobulin-related proteins expressed in hemocytes; 5) Studies on IRHP in Drosophila. The study of insect proteins related to immunoglobulins may yield information relevant to the understanding of insects as vectors of human and animal diseases and also contribute to knowledge on the evolution of the immunoglobulin gene superfamily.