课题基金 / 基金详情

STRUCTURE OF BRANCHED CHAIN KETO ACID DEHYDROGENASE

STRUCTURE OF BRANCHED CHAIN KETO ACID DEHYDROGENASE
支链酮酸脱氢酶的结构
批准号：
3151407
负责人：
JOHN R SOKATCH
金额：
$9.22万
依托单位：
UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
依托单位国家：
美国
项目类别：
财政年份：
1979
资助国家：
美国
项目状态：
已结题
起止时间：
1979-07-01 至 1987-06-30
来源：
https://reporter.nih.gov/project-details/3151407
关键词：
Pseudomonas aminoacid metabolism bacterial genetics biological models branched chain aminoacid cofactor electron microscopy enzyme complex enzyme induction /repression enzyme structure enzyme substrate complex gel electrophoresis gene expression genetic mapping maple syrup urine disease microorganism metabolism molecular weight mutant orphan disease /drug radiotracer stoichiometry

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中文摘要
翻译
恶臭假单胞菌支链酮脱氢酶是一种罕见的酶 其活性受L-缬氨酸的刺激。这种酶已经被提纯到一个 比活力为10个单位/mg蛋白质。纯化后的酶含有4个 亚菌落的相对分子质量分别为49K、46K、39K和37K。49K蛋白有 经鉴定为硫酰胺脱氢酶。该计划的目标是 本年度将分离和鉴定剩余的亚基。这个 转酰基酶亚基将通过其特定的标记与 2-酮基异戊酸酯存在下的N-乙基(2，3-14C)马来酰亚胺。这个 脱氢酶(E1)亚基将通过其与铁氰化物的反应来鉴定 和放射性二氧化碳从2-酮(1-14C)异戊酸酯的释放。
英文摘要
Branched chain keto dehydrogenase of Pseudomonas putida is an unusual enzyme whose activity is stimulated by L-valine. The enzyme has been purified to a specific activity of 10 units/mg protein. The purified enzyme contains 4 subunis with molecular weights of 49k, 46k, 39k, and 37k. The 49k protein has been isolated and identified as lipoamide dehydrogenase. The objective of the current year will be to isolate and identify the remainding subunits. The transacylase subunit will be identified by its specific labeling with N-ethyl(2,3-14C) maleimide in the presence of 2-ketoisovalerate. The dehydrogenase (E1) subunit will be identified by its reaction with ferricyanide and the release of radioactive carbon dioxide from 2-keto(1-14C) isovalerate.
期刊论文(3)
专著(0)
科研奖励(0)
会议论文
Purification of a branched-chain keto acid dehydrogenase from Pseudomonas putida.
从恶臭假单胞菌中纯化支链酮酸脱氢酶。
DOI： 10.1128/jb.148.2.647-652.1981
发表时间： 1981
期刊： Journal of bacteriology
影响因子： 3.2
作者： [Sokatch,JR, McCully,V, Roberts,CM]
通讯作者： Roberts,CM
Isolation of two lipoamide dehydrogenases from Pseudomonas putida grown on valine.
从在缬氨酸上生长的恶臭假单胞菌中分离出两种硫辛酰胺脱氢酶。
DOI： 10.1016/0006-291x(81)91932-x
发表时间： 1981
期刊： Biochemical and biophysical research communications
影响因子： 3.1
作者： [Sokatch,JR]
通讯作者： Sokatch,JR
Isolation of a specific lipoamide dehydrogenase for a branched-chain keto acid dehydrogenase from Pseudomonas putida.
从恶臭假单胞菌中分离支链酮酸脱氢酶的特定硫辛酰胺脱氢酶。
DOI： 10.1128/jb.148.2.639-646.1981
发表时间： 1981
期刊： Journal of bacteriology
影响因子： 3.2
作者： [Sokatch,JR, McCully,V, Gebrosky,J, Sokatch,DJ]
通讯作者： Sokatch,DJ
MINOTOME CRYOSTAT--CESCO GWCS 30 WASHER
KODAK M-35 FILM DEVELOPER/BECKMAN J2-21 CENTRIFUGE
BIOCHEMISTRY AND GENETICS OF LIPOAMIDE DEHYDROGENASE
BIOCHEMISTRY AND GENETICS OF LIPOAMIDE DEHYDROGENASE