CHARACTERIZATION OF THE IMMUNOREGULATORY CIRCUITS IN MAN

最近的研究表明，人类T细胞库是异质性的 尊重细胞表面标记和功能程序。此外， 包括控制HELP和HELP的调节电路的细胞 压制的定义仍然很模糊，尽管它们在许多 人类疾病，包括自身免疫和免疫缺陷疾病。我们 相信，鉴于最近在定义方面的重大技术突破 细胞表面标记、功能分析系统和克隆技术， 人们可以对免疫调节产生重要的新见解 巡回赛中的人。此外，我们刚刚开发了一套新的 确定T4抑制诱导因子亚群的单抗 (抗2H4抗体)和T4辅助诱导亚群(抗4B4抗体) 这将有助于解决T4细胞的异质性。至 描述人类的免疫调节回路，我们计划利用 最新研制的体外原代抗DNP抗体 系统。该系统允许对细胞-细胞进行精确检查 相互作用参与了抗DNP抗体抑制的产生 生产和表征释放的因素 免疫调节性T细胞。此外，我们计划开发KLH特异性T细胞 具有独特调节活动的细胞克隆。此外，我们计划 分析T细胞亚群、分离细胞的功能和FINE 自然产生的抗T细胞抗体在慢性支气管炎患者中的特异性 系统性红斑狼疮、幼年类风湿性关节炎和干燥综合征 疾病。我们认为对正常细胞和正常细胞的分析 自身免疫性疾病的患者将提供对 疾病的异质性和免疫调节的复杂性 巡回赛。希望能更好地了解监管 人类的回路对于理解这两种基本的 人类淋巴细胞的生物学和对人类淋巴细胞紊乱的了解 淋巴细胞存在于人类的多种疾病中。最后，它是 认为合理的治疗有赖于对 免疫调节回路。

Recent studies demonstrate that human T cell pool is heterogeneous with respect to cell surface markers and functional programs. Moreover, the cells which comprise the regulatory circuits controlling help and suppression are still poorly defined despite their importance in a host of human diseases, including autoimmune and immunodeficiency disorders. We believe, given the major recent technical breakthroughs in the definition of cell surface markers, functional assay systems, and cloning techniques, that one can develop important new insights into the immunoregulatory circuit in man. Furthermore, we have just developed a new set of monoclonal antibodies which define the T4 suppressor inducer subset (anti-2H4 antibody) and the T4 helper inducer subset (anti-4B4 antibody) that will help in the resolution of the heterogeneity of T4 cells. To characterize the immunoregulatory circuit in man, we plan to take advantage of the recently developed primary in vitro anti-DNP antibody forming system. This system allows for a precise examination of cell-cell interactions involved in the generation of supression of anti-DNP antibody production and for the characterization of the factors released by immunoregulatory T cells. Furthermore, we plan to develop KLH specific T cell clones which have unique regulatory activities. In addiiton, we plan to analyze the T cell subsets, functions of isolated cells, and fine specificity of naturally occurring anti-T cell antibodies in patients with systemic lupus erythematosus, juvenile rheumatoid arthritis, and Sjogren's disease. We believe that the analysis of both normal cells and cells from patients with autoimmune disease will provide new insights into the heterogeneity of the diseases and the complexity of the immunoregulatory circuit. It is hoped that a better understanding of the regulatory circuits in man will be critical for both an understanding of the basic biology of the human lymphocytes and for an understanding of disorders of lymphocytes which are present in a host of human diseases. Lastly, it is believed that rational therapy depends on the precise understanding of the immunoregulatory circuit.