INSULIN AND CATECHOLAMINE IN CARBOHYDRATE METABOLISM

该项目旨在研究糖原磷酸化酶的调节 在离体大鼠肝细胞中， α-儿茶酚胺 α-儿茶酚胺和加压素被认为 激活磷酸化酶继发于细胞内钙离子的升高。 我们 兴趣涉及α-肾上腺素能受体的性质， 对于α的所有代谢效应，属于α 1而不是α 2类别 儿茶酚胺 我们感兴趣的是， 在加入以下物质后，在肝细胞中观察到磷脂酰肌醇的周转： 加压素和儿茶酚胺以及胞浆钙的升高。 有 磷脂酰肌醇周转不增加继发于进入的证据 细胞外钙的浓度。 然而，这两个事件之间的联系仍然是 建立。 我们目前正在验证一个假设， 磷脂酰肌醇参与了导致 结合的细胞内钙的释放和细胞外钙的摄取。

The project is designed to investigate the regulation of glycogen phosphorylase in isolated rat hepatocytes by hormones with particular emphasis on insulin and alpha catecholamines. Alpha catecholamines and vasopressin are thought to activate phosphorylase secondary to an elevation of intracellular calcium. Our interests relate to the nature of the alpha-adrenergic receptors which appear to be of the alpha1 rather than alpha2 category for all metabolic effects of alpha catecholamines. We are interested in the relationship between the increased turnover of phosphatidylinositol seen in hepatocytes after the addition of vasopressin and catecholamines and the elevation of cytosol calcium. There is evidence that phosphatidylinositol turnover is not increased secondary to entry of extracellular calcium. However, the link between these two events remains to be established. We are currently testing the hypothesis that increased turnover of phosphatidylinositol is involved in the primary events which result in the release of bound intracellular calcium and the uptake of extracellular calcium.