ACTIN BASED REGULATION OF SMOOTH MUSCLE CONTRACTION

脊椎动物肌肉的收缩，像所有其他类型的肌肉一样 是肌球蛋白和肌动蛋白在体内循环作用的结果 与三磷酸腺苷的水解相耦合的过程。血管平滑肌的调节 收缩是非常复杂的，涉及几个不同的监管系统。 因为对平滑肌的调节非常重要 收缩，特别是与血管疾病有关的各种 必须确定这些监管系统的组成部分，并且 必须界定产生整体监管的相互作用。初级阶段 平滑肌中的调节系统似乎是以肌球蛋白为基础的 肌球蛋白去磷酸化导致松弛。更多的证据表明 累积肌动蛋白连接的系统可能会进一步调节力 产生肌球蛋白与肌动蛋白的相互作用。一位可能的候选人 肌动蛋白结合调节蛋白是最近发现的蛋白质， 卡尔德蒙。钙调蛋白的抑制功能将在#年研究。 细节。它与肌动蛋白和其他收缩蛋白的结合 仪器将被量化。最后，肌球蛋白与肌球蛋白之间的相互作用 本课程将研究基于肌动蛋白的平滑肌调控系统。其他 来自平滑肌的潜在调节蛋白也可能被研究。

The contraction of vertebrate smooth muscle, like all other types of muscle is the result of a cyclic interaction of the proteins myosin and actin in a process coupled to the hydrolysis of ATP. The regulation of smooth muscle contraction is very complex involving several distinct regulatory systems. Because of the great importance of the regulation of smooth muscle contraction, particularly in relation to vascular disease, the various components of these regulatory systems must be identified and the interactions which produce overall regulation must be defined. The primary regulatory system in smooth muscle appears to be myosin based with dephosphorylation of myosin causing relaxation. More evidence has been accumulating that an actin-linked system may further modulate the force producing interaction of myosin with actin. A likely candidate for an actin binding regulatory protein is the recently identified protein, caldesmon. The inhibitory function of caldesmon will be studied in detail. Its binding to actin and other proteins in the contractile apparatus will be quantitated. Finally, the interplay of myosin based and actin based regulatory systems in smooth muscle will be examined. Other potential regulatory proteins from smooth muscle may also be studied.