CONVERSION OF ORNITHINE, ARGININE, PROLINE AND GLUTAMATE

合成的酶和利用氨甲酰的代谢途径 磷酸盐的研究将主要在哺乳动物身上进行。这些研究的目的是 了解精氨酸(和尿素)和UMP途径的调节 生物合成。希望这样的尿素生物合成研究将是 对急性氨中毒很有用。在UMP生物合成的情况下 途径，很明显，这些酶的水平差异很大，因为 分化和发育，使快速分裂的组织，如 癌症、再生性和正常的肝脏、大脑等都有较高水平的这类物质。 酵素。我们还想看看这些酶是否能被“诱导”并 “被抑制”，或者如果水平升高仅仅是酶的结果 稳定状态。 研究将包括分离纯化和动力学表征。 两种途径的酶或UMP途径的酶复合体，并研究 关于这些哺乳动物酶在生长过程中的体内代谢控制， 分化，或当营养或激素状态发生变化时 正常个体以及对异常组织的研究，如癌症或 突变细胞。 粪链球菌和链球菌天冬氨酸氨基转移酶的研究 弗劳迪柠檬酸杆菌可能会继续，以及对激活的工作 酵母型碳酸酐酶。

The enzymes which synthesize and the metabolic pathways which utilize carbamyl phosphate will be studied principally in mammals. The aim of the studies is to understand the regulation of the pathways, namely, arginine (and urea) and UMP biosynthesis. It is hoped that such studies for urea biosynthesis will be useful in acute ammonia intoxication. In the case of the UMP biosynthetic pathway, it is clear that the levels of these enzymes vary greatly due both to differentiation and development, so that tissues which divide rapidly such as cancers, regenerating and normal liver, brain, etc., have high levels of these enzymes. We would also like to see if the enzymes can be "induced" and "repressed," or if increased levels are merely the result for enzyme stabilization. Studies will involve purification and kinetic characterization of isolated enzymes of both pathways or the enzyme complexes of the UMP pathway, and studies on the in vivo metabolic control of these mammalian enzymes during growth, differentiation, or when changes in nutritional or hormonal states occur in normal individual as well as studies of abnormal tissues such as cancer or mutant cells. Former work on the aspartate transcarbamylases of Streptococcus faecalis and Citrobacter freundii may be continued, as well as work on the activator of the yeast carbonic anhydrase.