BIOLOGICAL EFFECTS OF BASIC CALCIUM PHOSPHATE CRYSTALS

这些研究旨在确定该病的发病机制。 与骨沉积相关的退行性关节病 二水焦磷酸钙或碱性磷酸钙 关节组织中的晶体，最终目标是开发一种 以合理的方式防止或扭转 这样的证词。这项建议主要集中在互动上 含有含钙晶体的活细胞。 我们将研究观察到的生物效应，如 促进有丝分裂、晶体溶解、花生四烯酸 代谢和蛋白水解酶的合成是相互联系的。我们 应进一步明确BCP晶体诱导的机理 特别强调早期生化变化的有丝分裂 由晶体刺激的细胞。BCP晶体的生长机理 将探索细胞的溶解作用。我们对可能的可能性感兴趣 晶体产生的钙离子在细胞内的滞留 Ca~(2+)的溶解和外流速率。 其他相关研究将包括：人体内生长抑素C水平 关节炎患者关节液；2)BCP晶体的清除 来自人类的关节。 兔子和小鼠将被用作细胞的细胞来源 文化。

These studies are designed to determine the pathogenesis of the degenerative arthropathies associated with the deposition of calcium pyrophosphate dihydrate or basic calcium phosphate crystals in articular tissues with the eventual goal of developing a rational means of preventing or reversing the consequences of such deposition. This proposal focuses chiefly on the interaction of living cells with crystals containing calcium. We shall examine how the observed biological effects such as stimulation of mitogenesis, crystal dissolution, arachidonic acid metabolism and proteases synthesis are related to each other. We shall further define the mechanism of BCP crystal-induced mitogenesis with special emphasis on early biochemical changes in cells stimulated by crystals. The mechanism of BCP crystal dissolution by cells will be explored. We are interested in possible intracellular sequestration of Ca2+ resulting from crystal dissolution and rate of Ca2+ efflux. Other related studies will include: level of somatomedin C in joint fluids of arthritis patients; 2) clearance of BCP crystals from human joints. Rabbits and mice will be used as a source for cells for cell culture.