ANTITUMOR AGENTS FROM MARINE ORGANISMS

本研究的目的是识别、分离和确定 来自一种细胞毒性和体内肿瘤抑制化合物的结构 各种海洋生物。 新化合物的主要来源将是 海绵海鞘和腔肠动物 此外，海洋细菌 从海绵和一些其他海洋来源分离的细菌将被培养， 以寻找潜在的抗肿瘤化合物。 隔离将是 由国家癌症研究所提供的活动测试指导， 体内（PS）和/或体外（KB、P388、L1210）试验系统。 尽 可能的化合物将以足够的量被分离，以进行 体内活性测试。 将寻求抗肿瘤活性增强 通过对一些分离的活性化合物进行结构修饰。 将使用各种已建立的现代分离技术， 分离化合物和结构将通过单晶X射线 分析和/或常规光谱方法。 的结构 主要目的附带获得的化学新化合物 我们将尽可能进行调查。 该项目的长期目标是发现临床上有用的 抗癌剂。

The objectives of this research are to identify, isolate and determine the structure of cytotoxic and in vivo tumor inhibitory compounds from a variety of marine organisms. The primary sources for new compounds will be sponges, ascidians, and coelenterates. In addition, marine bacteria isolated from sponges and some other marine sources will be cultured and extracted to search for potential antitumor compounds. Isolation will be guided by activity testing provided by the National Cancer Institute using in vivo (PS) and/or in vitro (KB, P388, L1210) test systems. To the extent possible compounds will be isolated in sufficient quantities to conduct in vivo activity testing. Antitumor activity enhancement will be sought through structural modification of some of the active compounds isolated. A variety of established, modern separation techniques will be used to isolate compounds, and structures will be determined by single cystal x-ray analysis and/or conventional spectroscopic methods. The structures of chemically novel compounds obtained incidental to the primary objective will be investigated to the extent possible. The long term goal of this project is to discover clinically useful anticancer agents.