BIOCHEMICAL AND CELLULAR RESPONSES TO BIOMATERIALS

全关节置换术彻底改变了 终末期破坏性关节炎患者的治疗。 尽管生物材料和外科技术的进步 取得了良好的临床效果，长期随访研究 显示假体松动的速度令人震惊，最终 需要做翻修手术。尽管许多研究都描述了 与骨科植入物相关的组织反应，非常 关于特定的细胞和生物化学知识知之甚少。 决定组织学特征的事件和 结缔组织对这些材料的反应。这样做的目的是 建议开发一种体外细胞培养模型，利用 研究骨等结缔组织细胞的作用 骨科种植体生物材料对其表达的影响 分化的细胞功能。生化与分子 调节一般细胞活动的机制，如 附着、增殖、细胞骨架组织和基质 将对生物合成进行研究，并获得比较结果 在不同的细胞类型中的表达将有助于确定 用骨细胞进行的观察。此外，这款车型将是 用于探索实验方法来调制 骨和其他结缔组织细胞的表型表达 生长在植入物材料上。一种合适的体外模型 研究相互作用中涉及的特定生物过程 骨科生物材料与结缔组织细胞之间的关系 应该有助于开发改进的技术和 用于全关节置换的生物材料。

Procedures for total joint replacement have revolutionized the treatment of patients with end-stage destructive arthritis. Although improvements in biomaterials and surgical techniques have yielded excellent clinical results, long-term followup studies reveal an alarming rate of prosthetic loosening that ultimately requires revision surgery. Despite numerous studies describing the tissue reaction associated with orthopedic implants, very little is known regarding the specific cellular and biochemical events that determine the characteristics of the histologic and connective tissue response to these materials. The goal of this proposal is to develope an in vitro cell culture model employing bone and other connective tissue cells to study the effects of orthopaedic implant biomaterials on the expression of differentiated cell function. Biochemical and molecular mechanisms regulating general cellular activities such as attachment, proliferation, cytoskeletal organization and matrix biosynthesis will be investigated and comparative results obtained in different cell types will help establish the specificity of the observations made with bone cells. In addition this model will be used to explore experimental approaches for modulating the phenotypic expression of bone and other connective tissue cells grown on implant materials. A suitable in vitro model for studying the specific biologic processes involved in the interaction between orthopedic biomaterials and connective tissue cells should be useful in developing improved techniques and biomaterials for total joint replacement.