BIOLOGICAL EFFECTS OF BASIC CALCIUM PHOSPHATE CRYSTALS

这些研究旨在确定 退行性关节病与沉积的 焦磷酸钙二水合物或碱性磷酸钙 关节组织中的晶体，最终目标是发展一种 防止或扭转后果的合理手段 这样的沉积。 该建议主要侧重于互动 含有钙的晶体的活细胞 我们将研究观察到的生物效应，如 刺激有丝分裂，晶体溶解，花生四烯酸 代谢和蛋白酶的合成相互关联。 我们 应进一步定义BCP晶体诱导的机制 有丝分裂，特别强调早期生化变化， 晶体刺激的细胞。 嵌段共聚物结晶机理 将探索细胞的溶解。 我们感兴趣的是 晶体引起细胞内Ca ~（2+）螯合 溶解和Ca 2+流出速率。 其他相关研究将包括： 关节炎患者的关节液; 2）BCP晶体的清除 从人体关节中分离出来 兔和小鼠将被用作细胞的来源。 文化

These studies are designed to determine the pathogenesis of the degenerative arthropathies associated with the deposition of calcium pyrophosphate dihydrate or basic calcium phosphate crystals in articular tissues with the eventual goal of developing a rational means of preventing or reversing the consequences of such deposition. This proposal focuses chiefly on the interaction of living cells with crystals containing calcium. We shall examine how the observed biological effects such as stimulation of mitogenesis, crystal dissolution, arachidonic acid metabolism and proteases synthesis are related to each other. We shall further define the mechanism of BCP crystal-induced mitogenesis with special emphasis on early biochemical changes in cells stimulated by crystals. The mechanism of BCP crystal dissolution by cells will be explored. We are interested in possible intracellular sequestration of Ca2+ resulting from crystal dissolution and rate of Ca2+ efflux. Other related studies will include: level of somatomedin C in joint fluids of arthritis patients; 2) clearance of BCP crystals from human joints. Rabbits and mice will be used as a source for cells for cell culture.