T-LYMPHOCYTE REGULATION OF NEUTROPHILS (PMN) FUNCTION

淋巴细胞激素或淋巴因子的细胞间通讯是 有助于调节免疫反应，维持宿主 防御感染，防止自身反应，并提供监测 对抗肿瘤 对淋巴因子的研究将增加我们的 了解紊乱的免疫调节，并提供新的治疗方法 许多人类疾病的病原体。 每种淋巴因子的研究 需要包括纯化至均质的综合方法， 化学表征，生物功能评估， 单特异性抗体的产生和基因克隆。 本实验室 将中性粒细胞迁移抑制因子纯化至均一， （NIF-T），这是一种中性粒细胞活化因子产生的辅助 T淋巴细胞 这一成就确立了 实现剩下的目标。 1.建立嗜铬细胞毒体外实验模型 既依赖抗体又不依赖抗体。 2.为了确定NIF-T（一种中性粒细胞活化因子）对 嗜中性粒细胞介导的肿瘤细胞毒性。 3.为了克隆NIF-T基因，以提供大量的NIF-T， 实验室和临床研究。

Intercellular communication by lymphocyte hormones, or lymphokines, is instrumental in the regulation of immune responses that maintain host defense against infection, prevent autoreactivity, and provide surveillance against neoplasia. The investigation of lymphokines will increase our understanding of disordered immune regulation and provide new therapeutic agents for many human diseases. The investigation of each lymphokine requires a comprehensive approach including purification to homogeneity, chemical characterization, assessment of biological functions, the production of monospecific antibodies, and gene cloning. Our laboratory has purified to homogeneity a neutrophil migration inhibition factor, (NIF-T), which is a neutrophil activating factor produced by helper T-lymphocytes. This accomplishment establishes the feasibility of achieving the remaining goals. 1. To establish in vitro assay models of neutrophil-mediated cytotoxicity that are both antibody dependent and independent. 2. To determine the effect of NIF-T, a neutrophil activating factor, on neutrophil-mediated tumor cell cytotoxicity. 3. To clone the gene for NIF-T to provide abundant quantities of NIF-T for laboratory and clinical studies.