MEROCYANINE DYES AS LEUKEMIA SPECIFIC PROBES

我们的目标是使用独特的染料花菁540(MC540)来研究 造血细胞膜的构筑，以考察两者之间的关系 白血病细胞和正常细胞之间的关系，并探索 它在某些疾病的检测、诊断和治疗中的应用 白血病。 美蓝540(MC540)是一种结合荧光的有机分子 特别是白血病细胞的表面，从而使它们变得敏感 为光解干杯。因为染料的专一性跨越了血统和 物种边界，它必须确定对所有人来说都是基本的一些特征 白血病细胞。事实上，与人造脂泡的结合研究 而模型红细胞已经证明它能特异性地与膜结合 其中的脂类包装松散。在功能上，MC540结合膜 在红系细胞中通过去核参与受体的消除， 巨噬细胞的内吞作用，以及可能的受体消除或激活 在淋巴细胞中。 慢性粒细胞白血病患者红细胞的发现 用MC540进行白血病(CML)染色提供了一种简单的系统 不仅导致这些细胞染色的分子缺陷，而且 也称白血病粒细胞，因为它们共同起源于一种缺陷 干细胞，也可能被证明是遵循 慢性粒细胞白血病的临床病程。 初步结果表明，MC540不仅可以识别红细胞 来自慢性粒细胞白血病患者的红细胞，也来自于任何 我们测试过的其他骨髓增生性疾病：真性红细胞增多症， 骨髓纤维化，伴有髓样化生，原发性血小板增多症和 某些形式的急性髓系白血病。因此，染料的染色 使用MC540的红细胞可以提供一种简单的血液检测方法 一般的骨髓增生性疾病。(3)

The goals are to use the unique dye merocyanine 540 (MC540) to study the architecture of hematopoietic cell membranes, to examine the relationship between leukemia cells and their normal cell counterparts, and to explore its application in the detection, diagnosis, and treatment of certain leukemias. Merocyanine 540 (MC540) is a fluorescent organic molecule that binds specifically to the surfaces of leukemia cells and thereby sensitizes them to photolysis. Because specificity of the dye crosses both lineage and species boundaries, it must identify some feature fundamental to all leukemia cells. In fact, binding studies with artificial lipid vesicles and model erythrocytes have shown it to bind specifically to membranes in which the lipids are loosely packed. Functionally, MC540-binding membranes are involved in receptor elimination via enucleation in erythroid cells, endocytosis in macrophages, and possibly receptor elimination or activation in lymphocytes. The discovery that erythrocytes of patients with chronic myelogenous leukemia (CML) stain with MC540 offers a simple system in which to pursue the molecular defect responsible for staining of not only these cells, but also leukemic granulocytes because of their common origin in a defective stem cell, and may also prove a useful criterion with which to follow the course of CML in a clinical setting. Preliminary results indicate that MC540 can identify not only erythrocytes from patients with CML but also erythrocytes from patients with any of the other myeloproliferative diseases we have tested: polycythemia vera, myelofibrosis, with myeloid metaplasia, essential thrombocythemia and certain forms of acute myelogenous leukemia. Therefore, staining of erythrocytes with MC540 could provide a simple blood test for the detection of myeloproliferative diseases in general. (3)