T-LYMPHOCYTE REGULATED TUMOR CELL KILLING BY NEUTROPHILS

淋巴细胞激素或淋巴因子的细胞间通讯是 有助于调节维持宿主的免疫反应 防御感染，防止自身反应，并提供监控 抗肿瘤。淋巴因子的研究将增加我们的 认识免疫调节紊乱，提供新的治疗方法 治疗多种人类疾病的药物。各种淋巴因子的研究进展 需要一个全面的方法，包括对同质性的净化， 化学特性，生物功能的评估， 单特异性抗体的制备和基因克隆。我们的实验室 纯化了一种中性粒细胞迁移抑制因子， (NIF-T)，这是一种由Helper产生的中性粒细胞激活因子 T淋巴细胞。这一成就确立了 实现剩下的目标。 1.建立中性粒细胞介导的细胞毒性体外检测模型 它们既是抗体依赖的又是独立的。 2.检测中性粒细胞活化因子NIF-T对血管内皮细胞增殖的影响 中性粒细胞介导的肿瘤细胞杀伤作用。 3.克隆NIF-T基因，为NIF-T的高效表达奠定基础 实验室和临床研究。

Intercellular communication by lymphocyte hormones, or lymphokines, is instrumental in the regulation of immune responses that maintain host defense against infection, prevent autoreactivity, and provide surveillance against neoplasia. The investigation of lymphokines will increase our understanding of disordered immune regulation and provide new therapeutic agents for many human diseases. The investigation of each lymphokine requires a comprehensive approach including purification to homogeneity, chemical characterization, assessment of biological functions, the production of monospecific antibodies, and gene cloning. Our laboratory has purified to homogeneity a neutrophil migration inhibition factor, (NIF-T), which is a neutrophil activating factor produced by helper T-lymphocytes. This accomplishment establishes the feasibility of achieving the remaining goals. 1. To establish in vitro assay models of neutrophil-mediated cytotoxicity that are both antibody dependent and independent. 2. To determine the effect of NIF-T, a neutrophil activating factor, on neutrophil-mediated tumor cell cytotoxicity. 3. To clone the gene for NIF-T to provide abundant quantities of NIF-T for laboratory and clinical studies.