SELF-RENEWAL IN NORMAL/LEUKEMIC HEMOPOIETIC STEM CELLS

美洲商陆促分裂素条件培养基的纯化研究 脾淋巴细胞（SCM）已经表明，活性因子能够 刺激干细胞自我更新的是糖蛋白Multi-CSF（IL-3）。 一个 已经开发了可诱导的T淋巴细胞系（LB 3），其合成 大量的多重脑脊液 Multi-CSF的cDNA已从 从LB 3细胞构建的mRNA文库。 克隆的作用 将在三个细胞中分析Multi-CSF对干细胞自我更新的影响。 系统-正常骨髓干细胞，多CSF依赖性连续 多能细胞系和V-ras转化的造血细胞。 将继续研究G-CSF的作用机制， 抑制骨髓单核细胞白血病WEHI-3B中的干细胞自我更新， 并将尝试克隆这些细胞上的G-CSF受体。

Purification studies on medium conditioned by pokeweed mitogen-stimulated spleen lymphocytes (SCM) have shown that the active factor able to stimulate stem cell self-renewal is the glycoprotein Multi-CSF (IL-3). An inducible T-lymphocyte cell line (LB3) has been developed that synthesizes large amounts of Multi-CSF. The cDNA for Multi-CSF has been cloned from an mRNA library constructed from LB3 cells. The action of cloned Multi-CSF will be analyzed on the self-renewal of stem cells in three cell systems--normal marrow stem cells, Multi-CSF-dependent continuous multipotential cell lines, and v-ras-transformed hemopoietic cells. Studies will be continued on the mechanism of action of G-CSF in suppressing stem cell self-renewal in the myelomonocytic leukemia WEHI-3B, and attempts will be made to clone the receptor for G-CSF on these cells.