FC-GAMMA RECEPTOR-MEDIATED REGULATION OF MACROPHAGE

这项工作的总体目标是阐明分子机制 细胞间和细胞内的通讯， 免疫球蛋白G与Fc-γ受体（Fc-γ-R）的特异性结合 存在于B细胞、巨噬细胞和其它细胞类型的表面上。 的 具体目标是：（1）确定初始生化反应的性质 由鼠巨噬细胞表面上的Fc-γ-Rs传递的信号， 巨噬细胞系和（2）调查的生化序列 Fc-Fc-γ-R介导的初始信号后的事件 互动 这些目标将通过以下方式实现：（1）隔离， Fc-γ-Rs、磷脂酶A的表征2，腺苷酸环化酶， 和cAMP依赖性蛋白激酶和（2）Fc-γ-R-触发的 环氧合酶和脂氧合酶途径，cAMP合成和活化 cAMP依赖性蛋白激酶在细胞水平上。 获得的结果 应该有助于理解的生化基础， 在免疫反应中起重要作用的细胞相互作用， 将军 （简体中文）

The overall objective of the work is to elucidate the molecular mechanism of cell-cell and intracellular communications that are triggered by the specific binding of the immunoglobulin G to Fc-gamma receptors (Fc-gamma-R) present on the surface of B cells, macrophages, and other cell types. The specific aims are to: (1) determine the nature of an initial biochemical signal transmitted by Fc-gamma-Rs on the surface of murine macrophages and macrophage-cell lines and (2) investigate the biochemical sequence of events following the initial signal transmitted by the Fc-Fc-gamma-R interaction. These goals will be approached by: (1) isolation and characterization of Fc-gamma-Rs, phospholipase A2, adenylate cyclases, and cAMP-dependent protein kinases and (2) studies of Fc-gamma-R-triggered cyclooxygenase and lipooxygenase pathways, cAMP synthesis, and activation of cAMP-dependent protein kinases at the cell level. The results obtained should contribute to the understanding of the biochemical basis for the cellular interactions that play important roles in the immune response in general. (CS)