HUMAN LYMPHOCYTES CLONALLY TRANSFORMED BY EBV

人类易感淋巴细胞的精确个体发育范围 爱泼斯坦-巴尔病毒(EBV)的转化尚未确定。近期 但不完全的证据表明，EBV可能会改变一系列 B淋巴细胞表型，从“早期”(不产生免疫球蛋白)B细胞到 前B细胞和B细胞。免疫球蛋白的表达及细胞遗传学分析 Burkitt肿瘤细胞中的现象表明， 将它们与其他EBV转化的细胞区分开来 在他们的免疫球蛋白基因分化程序中的一个特殊阶段。这 致癌事件可能包括人c-myc基因易位。我们会 试图更好地定义人类淋巴细胞的个体发育范围 易受EBV介导的转化的。原代单个核细胞来源 人类胎儿、新生儿和成人组织将克隆转化为 琼脂糖基。克隆传播的品系将通过以下方式表型 免疫球蛋白表达、表面“Ia”抗原等的分析 表位。还将进行核型分析。然后细胞克隆就会 对其免疫球蛋白基因重排模式进行分析。细胞克隆与 未表达的免疫球蛋白基因等位基因的异常重排将被检测 寻找细胞c-myc基因或染色体重排的证据 易位。我们的研究结果应该会有一个更清晰的图景 人类(B-)淋巴细胞个体发育的哪些阶段易感 通过EBV进行转化。我们或许能部分地解释 临床恶性B细胞克隆出现于多克隆转化 人口。

The precise ontogenetic range of human lymphocytes susceptible to transformation by Epstein-Barr Virus (EBV) has not been defined. Recent but incomplete evidence suggests that EBV may transform a range of B-lymphocyte phenotypes, from "early" (non-Ig producing) B-cells through pre-B cells and B-cells. Analyses of Ig expression and cytogenetic phenomena in Burkitt tumor cells suggest that the oncogenic event which distinguishes them from other EBV-transformed cells may be associated with a particular stage in their Ig-gene differentiation program. This oncogenic event may include a human c-myc gene translocation. We will attempt to better define the ontogenetic range of human lymphocytes susceptible to EBV-mediated transformation. Primary mononuclear cells from human fetal, neonatal, and adult tissues will be clonally transformed in agarose medium. Clonally propagated lines will then be phenotyped by analysis of Ig expression, surface "Ia" antigen, and other surface epitopes. Karyotype analyses will also be done. The cell clones will then be analyzed for their patterns of Ig gene rearrangements. Cell clones with aberrant rearrangements of non-expressed Ig gene alleles will be examined for evidence of rearrangement of the cellular c-myc gene, or chromosomal translocation. The results of our studies should yield a clearer picture of the stages of human (B-) lymphocyte ontogeny which are susceptible to transformation by EBV. We may be able to partially illuminate how clinically malignant B-cell clones emerge from a polyclonally transformed population.