"EXPERIMENTAL RADIOTHERAPY, AND PROTECTORS"

本次调查的目标是放射生物学特征。 从实体瘤中分离和分离的肿瘤细胞群 转移的目的是最终将该信息整合到 开发一种预测肿瘤反应的方法。这项研究将集中在 肿瘤反应的内部和内部变化。跟随 单独或联合暴露于体外或原位辐射 对于其他方式，选定的肿瘤细胞亚群将被移除 并通过生物物理方法进行分离。他们的反应将被确定 并与肿瘤的整体反应进行比较。肿瘤系统 将进行研究的包括：一种甲基胆蒽诱导的纤维肉瘤 (FSA)及其体外生长克隆FSA 1233是自发产生的 能够形成自发转移的纤维肉瘤(NFSA)，以及 自发性乳腺癌(MCA-K)。细胞分离 使用的方法包括根据细胞的浮力来分离细胞 密度的线性密度梯度上的离心法 Renografin或Percoll，并根据其大小通过 离心式淘洗。每个分离的肿瘤种群将是 关于所选生物参数的特征 生化分析、同位素标记程序和流式细胞术 为了更好地了解它以前在 肿瘤。特别是，单独分离的细胞群体将是 在原位或体外暴露后进行研究，以确定：a) 对辐射的反应，b)对辐射增敏的反应 电子亲和药物，以及c)单独或间接辐射的年龄反应 与其他治疗药物联合使用。将这些发现应用于 一种预测肿瘤反应的方法--针吸活检的发展 方法将被开发出来。活检组织中细胞的密度分布 将与其相应来源的肿瘤的分布进行比较 演示此技术在评估亲属关系时的适用性 In中敏感和抗性、低氧或静止细胞的组成 原位肿瘤。这些结果将结合设计进行解释 最佳治疗方案。

The objective of this investigation is the radiobiological characterization of tumor cell populations separated and isolated from solid tumors and metastasis for the purpose of eventually integrating this information to develop a predictive assay for tumor response. This study will focus on both intra as well as inter variations in tumor response. Following exposure either in vitro or in situ to radiation alone or in combination with other modalities, selected tumor cell subpopulations will be removed and isolated by biophysical methods. Their responses will be determined and compared to the response of the tumor as a whole. The tumor systems that will be studied include: a methylcholanthrene-induced fibrosarcoma (FSa) and its in vitro growing clone, FSA 1233, a spontaneously arisen fibrosarcoma capable of forming spontaneous metastasis (NFSa), and a spontaneously arisen mammary carcinoma (MCa-K). The cell separation methods used include the separation of cells on the basis of their buoyant densities by means of centrifugation on linear density gradients of either Renografin or Percoll, and on the basis of their size by means of centrifugal elutriation. Each of the separated tumor populations will be characterized with respect to selected biological parameters using biochemical assays, isotope labeling procedures, and flow cytometry in order to better understand its previous environmental situation within the tumor. In particular, individual separated cell populations will be studied following either in situ or in vitro exposure to determine: a) response to radiation, b) response to radiation sensitizing electron-affinic drugs, and c) age response to radiation alone or in combination with other therapeutic agents. To apply these findings to the development of a predictive assay for tumor response, a needle biopsy method will be developed. Density distributions of cells from biopsies will be compared to distributions from their corresponding source tumors to demonstrate the applicability of this technique for evaluating the relative composition of sensitive and resistant, hypoxic or quiescent cells in in situ tumors. These results will be interpreted in relation to designing optimum therapeutic protocols.