THERMAL RESPONSE OF MAMMALIAN ZYGOTES AND GERM CELLS

这些实验将系统地研究热敏性， 产生耐热性的能力和诱导热的能力 早期哺乳动物受精卵体外休克蛋白合成 的 实验还将系统地研究热敏性 以及在哺乳动物生殖细胞中诱导耐热性的能力，如 通过表达在细胞中的显性致死突变的频率来测定， 哺乳动物体外受精卵。 我们的长期目标是应用独特的 通过加热哺乳动物受精卵和/或生殖细胞以 阐明参与热杀死的机制， 热耐受性和热休克蛋白的合成增强 细胞 将处于不同发育状态的小鼠受精卵加热， 在不同温度（39-43摄氏度）下的暴露时间长度。 存活率将通过它们完成卵裂和 成功形成囊胚。 加热和胚胎生长将在 体外 在分次给药实验中，将分2次给予热量 分别相隔4、8和24小时。 培养耐热性的能力 并诱导热休克蛋白合成的增强。 热对生殖细胞的损伤表现为显性热诱导， 致命突变 体外成功发育的频率 由加热的亲本产生的合子将被用作热诱导的指标。 损害 这将在不同的温度下进行测试， 处理和在不同的生殖细胞阶段。 这些实验将 提供哺乳动物受精卵和生殖细胞的热反应信息 细胞

These experiments will systematically investigate the thermal sensitivity, the ability to develop thermal tolerance and the ability to induce heat shock protein synthesis in early mammalian zygotes in vitro. The experiments also will systematically investigate the thermal sensitivity and the ability to induce thermal tolerance in mammalian germ cells, as assayed by the frequency of dominant lethal mutations expressed in mammalian zygotes in vitro. Our long term objective is to apply the unique aspects afforded by heating the mammalian zygote and/or the germ cell to elucidate the mechanisms involved in thermal killing, the induction of thermal tolerance and the enhanced synthesis of heat shock proteins in cells. Mouse zygotes in different developmental states will be heated for various lengths of exposure times at different temperatures (39-43 degrees C). Survival will be measured by their ability to complete cleavage and to successfully form a blastocyst. Heating and embryo growth will be in vitro. In split dose experiments, heat will be given in 2 fractions separated by 4, 8, and 24 hours. The ability to develop thermotolerance and to induce enhanced synthesis of heat shock proteins will be determined. Thermal damage to the germ cell is reflected by the induction of dominant lethal mutations. The frequency of successful development in vitro of zygotes sired by a heated parent will be used as the index of heat-induced damage. This will be tested at various temperatures, durations of the treatment and at different germ cell stages. These experiments will provide information on the thermal response of mammalian zygotes and germ cells.