IMMUNOLOGICAL HETEROGENEITY OF CEA

总的目标是确定抗原谱和免疫化学。 人消化系癌胚抗原的特性 系统，并研究CEA的相互关系，非特异性 交叉反应抗原(NCA)和两个CEA相关的胎粪抗原。vbl.使用 从常规抗血清中分离出针对独特和常见的抗体 CEA的特异性，血浆中CEA的免疫化学性质 以及来自正常人和恶性和非小细胞肺癌患者的组织 非恶性胃肠道疾病的特征将根据 大小和凝集素结合特性。物理化学，免疫化学， CEA样胎粪抗原的组织特异性将是 测定并与CEA和NCA进行比较。中的相互关系 将研究CEA和CEA样抗原在血浆和 组织标本。小鼠的单抗将被开发出来对抗 从结肠癌和胃癌中提取的CEA以及对 CEA类抗原进一步确定CEA和CEA的抗原特性 相关抗原。免疫化学图谱的更清晰的定义 CEA和相关抗原将使我们能够更全面地了解它们的 相互关系，并提供机会从以下方面对它们进行评估 与组织特异性、治疗反应、患者生存和癌症有关 细胞进化。(2)

The general objective is to define the antigenic profile and immunochemical characteristics of carcinoembryonic antigen (CEA) of the human digestive system and to study the interrelationships of CEA, nonspecific cross-reacting antigen (NCA), and two CEA-related meconium antigens. Using antibodies isolated from conventional antisera against unique and common specificities on CEA, the immunochemical nature of CEA appearing in plasma and tissue from normal individuals and patients with malignant and nonmalignant gastrointestinal disease will be characterized according to size and lectin binding properties. The physicochemical, immunochemical, and tissue specificity properties of the CEA-like meconium antigens will be determined and compared to those of CEA and NCA. The interrelationships in the expression of CEA and CEA-like antigens will be studied in plasma and tissue specimens. Murine monoclonal antibodies will be developed against CEA obtained from colonic and gastric cancer as well as against the CEA-like antigens to define further the antigenic specification of CEA and related antigens. A clearer definition of the immunochemical profile of CEA and related antigens would allow a more complete understanding of their interrelationships and provide opportunities to evaluate them with regard to tissue specificity, response to therapy, patient survival, and cancer cell evolution. (2)