SERUM TYROSYL KINASES IN HUMAN NEOPLASIA

酪氨酸特异性蛋白激酶是一种酶活性， 几种癌基因蛋白和生长因子受体。 蛋白质与此 罕见的活性已被证明会导致动物肿瘤， 在某些人类肿瘤中被放大 我们的初步研究表明， 血清是一种容易获得的人体标本，可用于研究 酪氨酸激酶在正常和肿瘤细胞中表达， 有机体 我们已经发现血清酪氨酰激酶活性是 在人类发育中表达，并且活性在 来自某些肿瘤性疾病患者的血清。 我们的目的是：（1）鉴定可能与酪氨酸激酶有关的血清酪氨酸激酶， 与人类肿瘤疾病;（2）\以确定血清酪氨酸激酶， 可用于癌症诊断试验;和（3）鉴定酪氨酰激酶 人类发展的不同阶段。 实现 这些目标，我们将断裂和表征不同的酪氨酰 人血清中的激酶种类。 血清酶与肝硬化的关系 生长因子受体和癌基因蛋白将通过以下方法建立： 生化比较和对特异性免疫反应性 试剂 这些血清酪氨酰激酶与以前的 鉴定的酶将被自磷酸化以鉴定活性 亚基分子量，测试糖基化，并用于制备 抗体的 将对特定的酪氨酰激酶和 它们在不同年龄组血清中的活性水平，以确定 在发育中表达的物种和来自患有 不同的肿瘤疾病，以确定相关的物种 肿瘤 酶的水平与阶段的相关性 该疾病将指示酪氨酰激酶测定在癌症中的价值 诊断. （一）

Tyrosine-specific protein kinase is an enzyme activity associated with several oncogene proteins and growth factor receptors. Proteins with this rare activity have been shown to cause tumors in animals and to be amplified in some human tumors. Our preliminary studies indicate that serum is an easily obtained human specimen which can be used to investigate tyrosyl kinases expressed in normal and neoplastic cells in the intact organism. We have found that serum tyrosyl kinase activity is developmentally expressed in humans and that the activity is elevated in sera from some patients with neoplastic disorders. Our aims are: (1)\to identify serum tyrosyl kinases that may be associated with human neoplastic disorders; (2)\to identify serum tyrosyl kinases that may be used in cancer diagnostic tests; and (3)\to identify tyrosyl kinases that are associated and different stages of human development. To achieve these aims we will fractionate and characterize the different tyrosyl kinase species in human serum. The relationship of serum enzymes with growth factor receptors and oncogene proteins will be established by biochemical comparisons and by reactivity to specific immunological reagents. Those serum tyrosyl kinases that are unrelated to previously identified enzymes will be autophosphorylated to identify the active subunit molecular weights, tested for glycosylation, and used to prepare antibodies. Comparisons will be made of the specific tyrosyl kinases and their levels of activity in sera from different age groups to define the species that are developmentally expressed and in sera from patients with different neoplastic disorders to define the species that are associated with neoplasia. Correlations of the levels of the enzyme with the stage of the disease will indicate the value of the tyrosyl kinase assay in cancer diagnosis. (1)