THERMAL RESPONSE OF MAMMALIAN ZYGOTES AND GERM CELLS

这些实验将系统地研究热敏感性， 发展耐热性和诱导受热的能力 早期哺乳动物受精卵体外休克蛋白的合成。这个 实验还将系统地研究热敏性。 以及在哺乳动物生殖细胞中诱导热耐受的能力，如 用显性致死突变的频率来分析 哺乳动物体外受精卵。我们的长期目标是将独特的 通过将哺乳动物受精卵和/或生殖细胞加热到 阐明热致死、诱导热致死的机制 耐热性与热休克蛋白的增强合成 细胞。 不同发育状态的小鼠受精卵将进行不同的加热 在不同温度(39-43摄氏度)下的曝光时间长度。 存活率将通过它们完成卵裂和 成功地形成了一个囊胚。加热和胚胎生长将在 体外培养。在分剂量实验中，热量将分成两份。 间隔4小时、8小时和24小时。发展耐热性的能力 并对诱导热休克蛋白合成增强进行了测定。 对生殖细胞的热损伤是通过诱导显性的 致命的突变。试管内发育成功的几率 由受热亲本产生的受精卵将作为受热诱导的指标。 损坏。这将在不同的温度、持续时间 治疗和处于不同生殖细胞阶段。这些实验将 提供有关哺乳动物受精卵和胚胎的热反应的信息 细胞。

These experiments will systematically investigate the thermal sensitivity, the ability to develop thermal tolerance and the ability to induce heat shock protein synthesis in early mammalian zygotes in vitro. The experiments also will systematically investigate the thermal sensitivity and the ability to induce thermal tolerance in mammalian germ cells, as assayed by the frequency of dominant lethal mutations expressed in mammalian zygotes in vitro. Our long term objective is to apply the unique aspects afforded by heating the mammalian zygote and/or the germ cell to elucidate the mechanisms involved in thermal killing, the induction of thermal tolerance and the enhanced synthesis of heat shock proteins in cells. Mouse zygotes in different developmental states will be heated for various lengths of exposure times at different temperatures (39-43 degrees C). Survival will be measured by their ability to complete cleavage and to successfully form a blastocyst. Heating and embryo growth will be in vitro. In split dose experiments, heat will be given in 2 fractions separated by 4, 8, and 24 hours. The ability to develop thermotolerance and to induce enhanced synthesis of heat shock proteins will be determined. Thermal damage to the germ cell is reflected by the induction of dominant lethal mutations. The frequency of successful development in vitro of zygotes sired by a heated parent will be used as the index of heat-induced damage. This will be tested at various temperatures, durations of the treatment and at different germ cell stages. These experiments will provide information on the thermal response of mammalian zygotes and germ cells.