STUDIES OF NORMAL AND NEOPLASTIC HUMAN PITUITARY TISSUE

尽管垂体瘤与许多疾病的发病率有关 由于荷尔蒙分泌过多和/或占位性病变， 人们对其发育的调控机制知之甚少 人类的脑下垂体增生和肿瘤。 正常人和正常人体内激素生成和分化的调节 人类脑下垂体肿瘤组织将使用 形态、免疫化学、生化和原位杂交 方法：研究方法。在光学和电子显微镜水平上的形态研究， 特异性抗体和原位杂交的免疫化学方法 将使用寡核苷酸分析、cDNA分析和核探针分析来研究 正常垂体腺、催乳素(PRL)、生长激素(GH)分泌及Null 细胞性或未分化腺瘤。细胞在细胞外基质上的培养 用无血清培养基将其用于分析特异性的效果 下丘脑激素，如促甲状腺激素释放激素、促性腺激素 促肾上腺皮质激素释放激素和生长抑素，AS 以及佛波酯对激素产生和分化的影响。受体 特定受体的放射自显影和生化分析将 在这些组织中进行，并与各种不同的 促分泌剂对细胞分化的影响。特定生长因子的作用， 如表皮生长因子和碱性成纤维细胞生长因子 将检查脑垂体细胞分化情况。雌激素的调节作用 受体、嗜铬颗粒蛋白和垂体转录因子信使RNA 我们将分析正常和肿瘤垂体腺细胞的水平。这个 长期目标是了解细胞的形态和生化 人正常脑垂体与肿瘤人脑垂体的异同 组织，并对正常和肿瘤的生物学有更多的了解 脑垂体细胞。来自这些研究的数据可能会带来更好的 对正常和肿瘤垂体腺细胞生物学的认识 人类和更多治疗这些肿瘤的方法的发展 有效地。

Although pituitary neoplasms are associated with a great deal of morbidity because of excessive hormone production and/or as space occupying lesions, very little is known about the mechanisms regulating the development of pituitary hyperplasia and neoplasia in humans. The regulation of hormone production and differentiation in normal and neoplastic human pituitary gland tissues will be investigated using morphological, immunochemical, biochemical and in situ hybridization methods. Morphological studies at the light and electron microscopic level, immunochemical methods with specific antibodies and in situ hybridization analyses with oligonucleotide, cDNA and riboprobes will be used to study normal pituitaries, prolactin (PRL), growth hormone (GH)-producing and null cell or undifferentiated adenomas. Cell culture on extracellular matrix with serum free media will be used to analyze the effects of specific hypothalamic hormones such as thyrotropin releasing hormone, gonadotropin releasing hormone, corticotropin releasing hormone and somatostatin, as well as phorbol esters on hormone production and differentiation. Receptor autoradiography and biochemical analysis for specific receptors will be performed in these tissues and correlated with the effects of various secretagogues on cell differentiation. The role of specific growth factors, such as epidermal growth factor and basic fibroblast growth factor on pituitary cell differentiation will be examined. The regulation of estrogen receptor, chromogranins and pituitary transcription factor messenger RNA levels in normal and neoplastic pituitary cells will be analyzed. The long-term objectives are to understand the morphological and biochemical differences and similarities between normal and neoplastic human pituitary tissues and to gain more insight into the biology of normal and neoplastic pituitary cells. The data from these studies may lead to a better understanding of the biology of normal and neoplastic pituitary cells in humans and to the development of methods to treat these tumors more effectively.