SEQUENTIAL 1H NMR STUDIES FOLLOWING CHEMOTHERAPY

这些研究的目的是调查和记录1H NMR 动物化疗和激素治疗后发生的变化， 人类肿瘤，并研究生物学变化，有助于 这些变化。 根据我们之前对这两种物质的核磁共振研究， 人类和动物肿瘤模型，这些NMR变化可能被证明是有用的， 快速评估特定治疗方案的疗效， 治疗对正常组织的毒理学， 基于NMR变化的生物学的时序治疗。 的 研究将包括一个大型肿瘤模型和一个 微转移肝模型，均为M5076肿瘤。 肿瘤核磁共振研究 体内和体外研究将与同时进行的 含水量、细胞外水、总脂质和细胞动力学 测量. 肿瘤生长过程中发生的变化和 将分析处理后，沿着的T1、T2和自旋密度的NMR变化。 肝转移瘤、骨转移瘤 化疗后测量T1和其他NMR参数 激素治疗将与临床和实验室相关 变化的证据。 描述相似变化的适当动物模型 将用于确定核磁共振变化的生物学原因， 准备使用这些参数进行非侵入性监测， 患者和设计治疗方案。

The purpose of these studies is to investigate and document the 1H NMR changes that occur after chemotherapy and hormonal therapy in animal and human tumors, and to investigate the biologic changes that contribute to these alterations. Based on prior NMR studies, performed by us, on both human and animal tumor models, these NMR changes may prove to be useful in rapidly assessing the efficacy of a particular treatment program, following the toxicology of treatment on normal tissues, and in designing time-sequential treatments based on the biology of NMR changes. The studies will include work with a large tumor mass model and a micrometactatic liver model, both of the M5076 tumor. Studies of tumor NMR in-vivo and in-vitro will be correlated with simultaneous studies of total water content, extracellular water, total lipids and cytokinetic measurements. Changes occuring during tumor growth and occuring during treatment will be analyzed, along with T1, T2 and spin density NMR changes. Sequential human in-vivo NMR studies of liver metastases, bone metastases, with measurements of T1 and other NMR parameters, following chemotherapy and hormonal therapy will be correlated with clinical and laboratory evidence of changes. Appropriate animal models describing similar changes will be used to identify the biologic reasons for the NMR changes, in preparation for using these parameters for non-invasive monitoring of patients and for designing treatment programs.