ANTICARCINOGENIC AGENTS IN ORANGE PEEL OIL

这里提出的研究试图定义使用的有效性 橙皮油抗癌作用机制的进一步研究 在这一角色中的行动。为了实现这一目标，高效液相技术将被用于 分离橙皮油的成分。黄连素的抗癌活性 这些成分将通过在完全定义的 DMBA染毒大鼠的饮食。致癌物的特异性将通过以下方式定义 橙皮油抗癌剂(S)抑癌能力的测定 包括自发性和诱发性乳腺肿瘤。器官特异性将是 经检测陈皮油抗癌剂(S)抗癌能力 抑制乳腺、皮肤和膀胱的肿瘤性变化。种 特异性研究将比较接触致癌物的大鼠的反应 和老鼠的橙油抗癌药物。此外，在体外类似 将使用大鼠和人的原代实质细胞进行比较 乳腺和肝脏。个体专一性将用RATE建模 对诱导乳腺具有高或低敏感性的菌株 癌症。所有动物实验将使用两个参数进行分析 和非参数统计程序。一旦抗癌药物 确定其药代动力学和代谢情况将予以界定。这个 这些代谢物的生物活性将被测定。这个 陈皮油抗癌剂(S)和/或其作用机理(英文) 将对代谢物进行研究。

The research proposed here seeks to define the efficacy of the use of orange peel oil as an anticarcinogen while further defining its mechanism of action in this role. To accomplish this HPLC techniques will be used to isolate the components of orange peel oil. The anticarcinogenic potency of these components will be quantitated by feeding them in a totally defined diet to rats exposed to DMBA. Carcinogen specificity will be defined by testing the ability of the orange peel oil anticarcinogen(s) to inhibit both spontaneous and induced mammary tumors. Organ specificity will be examined by testing the ability of the orange peel oil anticarcinogen(s) to inhibit neoplastic changes in mammary gland, skin and bladder. Species specificity studies will compare the response of carcinogen exposed rats and mice to orange oil anticarcinogens. In addition, similar in vitro comparisons will be made using rat and human primary parenchymal cells from mammary gland and liver. Individual specificity will be modeled with rat strains possessing high or low sensitivity to the induction of mammary carcinomas. All animal experiments will be analyzed using both parametric and nonparametric statistical procedures. Once the anticarcinogen is identified its pharmacokinetics and metabolism will be defined. The biological activity of these metabolites will be determined. The mechanism(s) of action of the orange peel oil anticarcinogen(s) and/or its metabolites will be studied.